Induction by verapamil of a rapid increase in ATP consumption in multidrug-resistant tumor cells

H. J. Broxterman, H. M. Pinedo, C. M. Kuiper, L. C M Kaptein, G. J. Schuurhuis, J. Lankelma

Research output: Contribution to journalArticlepeer-review

Abstract

A marked increase in cellular ATP consumption was induced by verapamil in the multidrug-resistant (MDR) cell line 2780(AD), but not in the drug-sensitive parental cell line A2780. A group of structurally unrelated drugs in concentrations known to reverse MDR, but not the verapamil analog tiapamil, a weak modulator of MDR, had similar effects. This effect was saturated at verapamil concentrations of about 1 μM. These data demonstrate that verapamil concentrations in MDR cells are maintained at a low level at the expense of ATP hydrolysis, and provide a first indication of the amount of metabolic energy used in this process.

Original languageEnglish (US)
Pages (from-to)2278-2282
Number of pages5
JournalFASEB Journal
Volume2
Issue number7
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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