The induction and repair of cyclobuta-dithymidine (T<≫T) photoproducts in hamster skin was measured following exposure to artificial solar radiation. DNA extracted from irradiated hamster epidermis was analyzed by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody specific for T<≫T photoproducts. Between 49% and 58% of T<≫T antibody binding sites were eliminated from DNA by 24 h after radiation exposures comparable with approximately 60 - 120 min of midday sunlight, whereas all detectable T<≫T binding sites were eliminated by 24 h after a radiation exposure comparable with 30 min of sunlight.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Photochemistry and Photobiology, B: Biology|
|State||Published - Feb 1989|
- DNA repair
- Solar simulator
- cyclobuta-dithymidine photoproducts
- enzyme-linked immunosorbent assay.
ASJC Scopus subject areas
- Radiological and Ultrasound Technology
- Radiology Nuclear Medicine and imaging