Induction and repair of cyclobuta-dithymidine photoproducts in hamster skin by solar-simulated UV radiation

Paul T. Strickland, Jessica S. Creasey

Research output: Contribution to journalArticle

Abstract

The induction and repair of cyclobuta-dithymidine (T<≫T) photoproducts in hamster skin was measured following exposure to artificial solar radiation. DNA extracted from irradiated hamster epidermis was analyzed by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody specific for T<≫T photoproducts. Between 49% and 58% of T<≫T antibody binding sites were eliminated from DNA by 24 h after radiation exposures comparable with approximately 60 - 120 min of midday sunlight, whereas all detectable T<≫T binding sites were eliminated by 24 h after a radiation exposure comparable with 30 min of sunlight.

Original languageEnglish (US)
Pages (from-to)17-24
Number of pages8
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume3
Issue number1
DOIs
StatePublished - Feb 1989

Keywords

  • DNA repair
  • Solar simulator
  • cyclobuta-dithymidine photoproducts
  • enzyme-linked immunosorbent assay.

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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