Induction and measurement of cytotoxic T lymphocyte activity

John Wonderlich, Gene Shearer, Alexandra Livingstone, Andrew Brooks, Mark J. Soloski, Matthew M. Presby

Research output: Contribution to journalArticlepeer-review

Abstract

Cytotoxic T cells (CTLs) are important immune effector cells in the adaptive immune response. It has been well documented that CTLs are important in host immune responses to viral and bacterial intracellular pathogens, tumors, and transplanted tissues. The properties of CTLs have been studied extensively in murine models, and their roles validated in the human setting. Frequently, the presence of these cells correlates well with protective immunity, so the ability to readily measure the activity of these cells is an important immunological measurement. In this unit, several assays are described that are commonly utilized to induce CTLs and to measure CTL activity both in vitro and in vivo. These assays are adaptable to many experimental and/or disease models, and in the case of the in vitro assays can be applied to measure CTL activity in human samples.

Original languageEnglish (US)
Pages (from-to)3.11.1-3.11.29
JournalCurrent protocols in immunology
Volume2018
DOIs
StatePublished - Feb 1 2018

Keywords

  • CTLs
  • Cr-release
  • Cytotoxic T cells
  • In vitro assays
  • In vivo assays
  • T cell clones
  • Target cells

ASJC Scopus subject areas

  • Immunology

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