Abstract
Cytotoxic T cells (CTLs) are important immune effector cells in the adaptive immune response. It has been well documented that CTLs are important in host immune responses to viral and bacterial intracellular pathogens, tumors, and transplanted tissues. The properties of CTLs have been studied extensively in murine models, and their roles validated in the human setting. Frequently, the presence of these cells correlates well with protective immunity, so the ability to readily measure the activity of these cells is an important immunological measurement. In this unit, several assays are described that are commonly utilized to induce CTLs and to measure CTL activity both in vitro and in vivo. These assays are adaptable to many experimental and/or disease models, and in the case of the in vitro assays can be applied to measure CTL activity in human samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3.11.1-3.11.29 |
| Journal | Current Protocols in Immunology |
| Volume | 2018 |
| DOIs | |
| State | Published - Feb 1 2018 |
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Keywords
- Cr-release
- CTLs
- Cytotoxic T cells
- In vitro assays
- In vivo assays
- T cell clones
- Target cells
ASJC Scopus subject areas
- Medicine(all)
Cite this
Induction and measurement of cytotoxic T lymphocyte activity. / Wonderlich, John; Shearer, Gene; Livingstone, Alexandra; Brooks, Andrew; Soloski, Mark J; Presby, Matthew M.
In: Current Protocols in Immunology, Vol. 2018, 01.02.2018, p. 3.11.1-3.11.29.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction and measurement of cytotoxic T lymphocyte activity
AU - Wonderlich, John
AU - Shearer, Gene
AU - Livingstone, Alexandra
AU - Brooks, Andrew
AU - Soloski, Mark J
AU - Presby, Matthew M.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Cytotoxic T cells (CTLs) are important immune effector cells in the adaptive immune response. It has been well documented that CTLs are important in host immune responses to viral and bacterial intracellular pathogens, tumors, and transplanted tissues. The properties of CTLs have been studied extensively in murine models, and their roles validated in the human setting. Frequently, the presence of these cells correlates well with protective immunity, so the ability to readily measure the activity of these cells is an important immunological measurement. In this unit, several assays are described that are commonly utilized to induce CTLs and to measure CTL activity both in vitro and in vivo. These assays are adaptable to many experimental and/or disease models, and in the case of the in vitro assays can be applied to measure CTL activity in human samples.
AB - Cytotoxic T cells (CTLs) are important immune effector cells in the adaptive immune response. It has been well documented that CTLs are important in host immune responses to viral and bacterial intracellular pathogens, tumors, and transplanted tissues. The properties of CTLs have been studied extensively in murine models, and their roles validated in the human setting. Frequently, the presence of these cells correlates well with protective immunity, so the ability to readily measure the activity of these cells is an important immunological measurement. In this unit, several assays are described that are commonly utilized to induce CTLs and to measure CTL activity both in vitro and in vivo. These assays are adaptable to many experimental and/or disease models, and in the case of the in vitro assays can be applied to measure CTL activity in human samples.
KW - Cr-release
KW - CTLs
KW - Cytotoxic T cells
KW - In vitro assays
KW - In vivo assays
KW - T cell clones
KW - Target cells
UR - http://www.scopus.com/inward/record.url?scp=85042525013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042525013&partnerID=8YFLogxK
U2 - 10.1002/cpim.38
DO - 10.1002/cpim.38
M3 - Article
C2 - 29512145
AN - SCOPUS:85042525013
VL - 2018
SP - 3.11.1-3.11.29
JO - Current Protocols in Immunology
JF - Current Protocols in Immunology
SN - 1934-3671
ER -