Inducible genetic suppression of neuronal excitability

David C. Johns, Ruth Marx, Richard E. Mains, Brian O'Rourke, Eduardo Marbán

Research output: Contribution to journalArticlepeer-review

Abstract

Graded, reversible suppression of neuronal excitability represents a logical goal of therapy for epilepsy and intractable pain. To achieve such suppression, we have developed the means to transfer 'electrical silencing' genes into neurons with sensitive control of transgene expression. An ecdysone-inducible promoter drives the expression of inwardly rectifying potassium channels in polycistronic adenoviral vectors. Infection of superior cervical ganglion neurons did not affect normal electrical activity but suppressed excitability after the induction of gene expression. These experiments demonstrate the feasibility of controlled ion channel expression after somatic gene transfer into neurons and serve as the prototype for a novel generalizable approach to modulate excitability.

Original languageEnglish (US)
Pages (from-to)1691-1697
Number of pages7
JournalJournal of Neuroscience
Volume19
Issue number5
DOIs
StatePublished - Mar 1 1999

Keywords

  • Adenovirus
  • Gene therapy
  • Genetic
  • Inducible expression
  • Neuronal excitability
  • Vital gene transfer

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Inducible genetic suppression of neuronal excitability'. Together they form a unique fingerprint.

Cite this