Inducibility of κ immunoglobulin enhancer-binding protein NF-κB by a posttranslational mechanism

Ranjan Sen, David Baltimore

Research output: Contribution to journalArticlepeer-review

Abstract

NF-κB is a nuclear protein, found only in cells that transcribe immunoglobulin light chain genes, that interacts with a defined site in the κ immunoglobulin enhancer. This protein can be induced in pre-B cells by stimulation with bacterial lipopolysaccharide (LPS). The induction involves a posttranslational activation, and the combined action of LPS and cycloheximide causes a superinduction. An active phorbol ester also induces this factor, and with kinetics more rapid than those for LPS stimulation. Phorbol-ester-mediated induction of NF-κB was observed in a T cell line (Jurkat) and a nonlymphoid cell line (HeLa), and is therefore not restricted to B-lymphoid cells. We interpret these results to indicate that factors that control transcription of specific genes in specific cells may be activated by posttranslational modification of precursor factors present more widely.

Original languageEnglish (US)
Pages (from-to)921-928
Number of pages8
JournalCell
Volume47
Issue number6
DOIs
StatePublished - Dec 26 1986
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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