Induced pluripotent stem cell model recapitulates pathologic hallmarks of Gaucher disease

Leelamma M. Panicker, Diana Miller, Tea Soon Park, Brijesh Patel, Judi L. Azevedo, Ola Awad, M. Athar Masood, Timothy D. Veenstra, Ehud Goldin, Barbara K. Stubblefield, Nahid Tayebi, Swamy K. Polumuri, Stefanie N. Vogel, Ellen Sidransky, Elias Zambidis, Ricardo A. Feldman

Research output: Contribution to journalArticle

Abstract

Gaucher disease (GD) is an autosomal recessive disorder caused by mutations in the acid β-glucocerebrosidase gene. To model GD, we generated human induced pluripotent stem cells (hiPSC), by reprogramming skin fibroblasts from patients with type 1 (N370S/N370S), type 2 (L444P/RecNciI), and type 3 (L444P/L444P) GD. Pluripotency was demonstrated by the ability of GD hiPSC to differentiate to all three germ layers and to form teratomas in vivo. GD hiPSC differentiated efficiently to the cell types most affected in GD, i.e., macrophages and neuronal cells. GD hiPSC-macrophages expressed macrophage-specific markers, were phagocytic, and were capable of releasing inflammatory mediators in response to LPS. Moreover, GD hiPSC-macrophages recapitulated the phenotypic hallmarks of the disease. They exhibited low glucocerebrosidase (GC) enzymatic activity and accumulated sphingolipids, and their lysosomal functions were severely compromised. GD hiPSC-macrophages had a defect in their ability to clear phagocytosed RBC, a phenotype of tissue-infiltrating GD macrophages. The kinetics of RBC clearance by types 1, 2, and 3 GD hiPSC-macrophages correlated with the severity of the mutations. Incubation with recombinant GC completely reversed the delay in RBC clearance from all three types of GD hiPSC-macrophages, indicating that their functional defects were indeed caused by GC deficiency. However, treatment of induced macrophages with the chaperone isofagomine restored phagocytosed RBC clearance only partially, regardless of genotype. These findings are consistent with the known clinical efficacies of recombinant GC and isofagomine. We conclude that cell types derived from GD hiPSC can effectively recapitulate pathologic hallmarks of the disease.

Original languageEnglish (US)
Pages (from-to)18054-18059
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number44
DOIs
StatePublished - Oct 30 2012

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Gaucher Disease
Induced Pluripotent Stem Cells
Macrophages
Glucosylceramidase
Phagocytosis
Germ Layers
Mutation
Sphingolipids
Teratoma

Keywords

  • Erythrophagocytosis
  • Gaucher macrophages
  • Gaucher model
  • Glucosylsphingolipids
  • Lipid storage disease

ASJC Scopus subject areas

  • General

Cite this

Induced pluripotent stem cell model recapitulates pathologic hallmarks of Gaucher disease. / Panicker, Leelamma M.; Miller, Diana; Park, Tea Soon; Patel, Brijesh; Azevedo, Judi L.; Awad, Ola; Masood, M. Athar; Veenstra, Timothy D.; Goldin, Ehud; Stubblefield, Barbara K.; Tayebi, Nahid; Polumuri, Swamy K.; Vogel, Stefanie N.; Sidransky, Ellen; Zambidis, Elias; Feldman, Ricardo A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 44, 30.10.2012, p. 18054-18059.

Research output: Contribution to journalArticle

Panicker, LM, Miller, D, Park, TS, Patel, B, Azevedo, JL, Awad, O, Masood, MA, Veenstra, TD, Goldin, E, Stubblefield, BK, Tayebi, N, Polumuri, SK, Vogel, SN, Sidransky, E, Zambidis, E & Feldman, RA 2012, 'Induced pluripotent stem cell model recapitulates pathologic hallmarks of Gaucher disease', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 44, pp. 18054-18059. https://doi.org/10.1073/pnas.1207889109
Panicker, Leelamma M. ; Miller, Diana ; Park, Tea Soon ; Patel, Brijesh ; Azevedo, Judi L. ; Awad, Ola ; Masood, M. Athar ; Veenstra, Timothy D. ; Goldin, Ehud ; Stubblefield, Barbara K. ; Tayebi, Nahid ; Polumuri, Swamy K. ; Vogel, Stefanie N. ; Sidransky, Ellen ; Zambidis, Elias ; Feldman, Ricardo A. / Induced pluripotent stem cell model recapitulates pathologic hallmarks of Gaucher disease. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 44. pp. 18054-18059.
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abstract = "Gaucher disease (GD) is an autosomal recessive disorder caused by mutations in the acid β-glucocerebrosidase gene. To model GD, we generated human induced pluripotent stem cells (hiPSC), by reprogramming skin fibroblasts from patients with type 1 (N370S/N370S), type 2 (L444P/RecNciI), and type 3 (L444P/L444P) GD. Pluripotency was demonstrated by the ability of GD hiPSC to differentiate to all three germ layers and to form teratomas in vivo. GD hiPSC differentiated efficiently to the cell types most affected in GD, i.e., macrophages and neuronal cells. GD hiPSC-macrophages expressed macrophage-specific markers, were phagocytic, and were capable of releasing inflammatory mediators in response to LPS. Moreover, GD hiPSC-macrophages recapitulated the phenotypic hallmarks of the disease. They exhibited low glucocerebrosidase (GC) enzymatic activity and accumulated sphingolipids, and their lysosomal functions were severely compromised. GD hiPSC-macrophages had a defect in their ability to clear phagocytosed RBC, a phenotype of tissue-infiltrating GD macrophages. The kinetics of RBC clearance by types 1, 2, and 3 GD hiPSC-macrophages correlated with the severity of the mutations. Incubation with recombinant GC completely reversed the delay in RBC clearance from all three types of GD hiPSC-macrophages, indicating that their functional defects were indeed caused by GC deficiency. However, treatment of induced macrophages with the chaperone isofagomine restored phagocytosed RBC clearance only partially, regardless of genotype. These findings are consistent with the known clinical efficacies of recombinant GC and isofagomine. We conclude that cell types derived from GD hiPSC can effectively recapitulate pathologic hallmarks of the disease.",
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AU - Miller, Diana

AU - Park, Tea Soon

AU - Patel, Brijesh

AU - Azevedo, Judi L.

AU - Awad, Ola

AU - Masood, M. Athar

AU - Veenstra, Timothy D.

AU - Goldin, Ehud

AU - Stubblefield, Barbara K.

AU - Tayebi, Nahid

AU - Polumuri, Swamy K.

AU - Vogel, Stefanie N.

AU - Sidransky, Ellen

AU - Zambidis, Elias

AU - Feldman, Ricardo A.

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