TY - JOUR
T1 - Induced Pluripotent Stem Cell-derived Mesenchymal Stem Cell Seeding on Biofunctionalized Calcium Phosphate Cements
AU - Theinhan, Wahwah
AU - Liu, Jun
AU - Tang, Minghui
AU - Chen, Wenchuan
AU - Cheng, Linzhao
AU - Xu, Hockin H.K.
N1 - Funding Information:
We thank Bin-Kuan Chou for experimental help with iPSCs, and Dr. Ferenc Livak for help with flow cytometry which were performed at the University of Maryland Greenbaum Cancer Center Shared Flow Cytometry Facility. We also thank Drs. Michael D. Weir and Ping Wang of the University of Maryland School of Dentistry for helpful discussions. This study was supported by NIH R01 DE14190 (HX), R21 DE22625 (HX) and R01 HL-073781 (LC), and the University of Maryland School of Dentistry startup fund (HX). There is no conflict of interest.
PY - 2013/12/31
Y1 - 2013/12/31
N2 - Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferation and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34+ cells which were reprogrammed by a single episomal vector. iPSCs were cultured to form embryoid bodies (EBs), and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC: RGD (Arg-Gly-Asp) peptides, fibronectin (Fn), fibronectin-like engineered polymer protein (FEPP), extracellular matrix Geltrex, and platelet concentrate. iPSC-MSCs were seeded on five biofunctionalized CPCs: CPC-RGD, CPC-Fn, CPC-FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and collagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering. iPSC-MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/orthopedic repairs.
AB - Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferation and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34+ cells which were reprogrammed by a single episomal vector. iPSCs were cultured to form embryoid bodies (EBs), and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC: RGD (Arg-Gly-Asp) peptides, fibronectin (Fn), fibronectin-like engineered polymer protein (FEPP), extracellular matrix Geltrex, and platelet concentrate. iPSC-MSCs were seeded on five biofunctionalized CPCs: CPC-RGD, CPC-Fn, CPC-FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and collagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering. iPSC-MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/orthopedic repairs.
KW - RGD
KW - biofunctionalized calcium phosphate cement
KW - bone tissue engineering
KW - fibronectin
KW - induced pluripotent stem cells
KW - platelet concentrate
UR - http://www.scopus.com/inward/record.url?scp=84902547483&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902547483&partnerID=8YFLogxK
U2 - 10.4248/BR201304008
DO - 10.4248/BR201304008
M3 - Article
AN - SCOPUS:84902547483
SN - 2095-4700
VL - 1
SP - 371
EP - 384
JO - Bone Research
JF - Bone Research
ER -