Indoleamine 2,3-Dioxygenase and Foxp3 Expression in Skin Rejection of Human Hand Allografts

T. Hautz, Gerald Brandacher, B. Zelger, H. G. Müller, W P Andrew Lee, D. Fuchs, R. Margreiter, S. Schneeberger

Research output: Contribution to journalArticle

Abstract

Human hand transplantation is complicated by skin rejection. To better define the characteristics of infiltrating cells, biopsies from human hand transplants have been investigated for expression of Foxp3 and indoleamine 2,3-dioxygenase (IDO), a key regulatory enzyme to induce T-lymphocyte unresponsiveness. A total of 104 skin biopsies taken from three bilateral hand transplant recipients over 6 years posttransplant were assessed by hematoxylin-eosin histology (graded 1-4b) and immunohistochemistry for IDO and Foxp3 according to a three-grade classification and correlated with the grade of rejection as well as time after transplantation. Overall, rejection ranged between grades 0 and 4a with an average score of 0.94. IDO was expressed in the endothelium independent of rejection. Upon rejection, IDO staining within the cellular infiltrate was significantly increased. Foxp3 in regulatory T cells was mainly found in samples undergoing severe rejection. Expression of IDO and Foxp3 compared well to each other, although the overall expression of Foxp3 was lower when compared to IDO. An increased expression of IDO as well as Foxp3 during rejection late after transplantation was observed. Characteristics of the cellular infiltrate indicate tolerogenic properties of a proportion of the cells and therefore a tendency toward self-limitation of the alloimmune response during skin rejection after hand transplantation.

Original languageEnglish (US)
Pages (from-to)509-512
Number of pages4
JournalTransplantation Proceedings
Volume41
Issue number2
DOIs
StatePublished - Mar 2009
Externally publishedYes

Fingerprint

Indoleamine-Pyrrole 2,3,-Dioxygenase
Allografts
Hand
Skin
Hand Transplantation
Transplantation
Biopsy
Regulatory T-Lymphocytes
Hematoxylin
Eosine Yellowish-(YS)
Endothelium
Histology
Immunohistochemistry
Staining and Labeling
T-Lymphocytes
Transplants
Enzymes

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Indoleamine 2,3-Dioxygenase and Foxp3 Expression in Skin Rejection of Human Hand Allografts. / Hautz, T.; Brandacher, Gerald; Zelger, B.; Müller, H. G.; Lee, W P Andrew; Fuchs, D.; Margreiter, R.; Schneeberger, S.

In: Transplantation Proceedings, Vol. 41, No. 2, 03.2009, p. 509-512.

Research output: Contribution to journalArticle

Hautz, T, Brandacher, G, Zelger, B, Müller, HG, Lee, WPA, Fuchs, D, Margreiter, R & Schneeberger, S 2009, 'Indoleamine 2,3-Dioxygenase and Foxp3 Expression in Skin Rejection of Human Hand Allografts', Transplantation Proceedings, vol. 41, no. 2, pp. 509-512. https://doi.org/10.1016/j.transproceed.2009.01.008
Hautz, T. ; Brandacher, Gerald ; Zelger, B. ; Müller, H. G. ; Lee, W P Andrew ; Fuchs, D. ; Margreiter, R. ; Schneeberger, S. / Indoleamine 2,3-Dioxygenase and Foxp3 Expression in Skin Rejection of Human Hand Allografts. In: Transplantation Proceedings. 2009 ; Vol. 41, No. 2. pp. 509-512.
@article{90e9f666a1bd4bb2b0590211b80b8f1c,
title = "Indoleamine 2,3-Dioxygenase and Foxp3 Expression in Skin Rejection of Human Hand Allografts",
abstract = "Human hand transplantation is complicated by skin rejection. To better define the characteristics of infiltrating cells, biopsies from human hand transplants have been investigated for expression of Foxp3 and indoleamine 2,3-dioxygenase (IDO), a key regulatory enzyme to induce T-lymphocyte unresponsiveness. A total of 104 skin biopsies taken from three bilateral hand transplant recipients over 6 years posttransplant were assessed by hematoxylin-eosin histology (graded 1-4b) and immunohistochemistry for IDO and Foxp3 according to a three-grade classification and correlated with the grade of rejection as well as time after transplantation. Overall, rejection ranged between grades 0 and 4a with an average score of 0.94. IDO was expressed in the endothelium independent of rejection. Upon rejection, IDO staining within the cellular infiltrate was significantly increased. Foxp3 in regulatory T cells was mainly found in samples undergoing severe rejection. Expression of IDO and Foxp3 compared well to each other, although the overall expression of Foxp3 was lower when compared to IDO. An increased expression of IDO as well as Foxp3 during rejection late after transplantation was observed. Characteristics of the cellular infiltrate indicate tolerogenic properties of a proportion of the cells and therefore a tendency toward self-limitation of the alloimmune response during skin rejection after hand transplantation.",
author = "T. Hautz and Gerald Brandacher and B. Zelger and M{\"u}ller, {H. G.} and Lee, {W P Andrew} and D. Fuchs and R. Margreiter and S. Schneeberger",
year = "2009",
month = "3",
doi = "10.1016/j.transproceed.2009.01.008",
language = "English (US)",
volume = "41",
pages = "509--512",
journal = "Transplantation Proceedings",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "2",

}

TY - JOUR

T1 - Indoleamine 2,3-Dioxygenase and Foxp3 Expression in Skin Rejection of Human Hand Allografts

AU - Hautz, T.

AU - Brandacher, Gerald

AU - Zelger, B.

AU - Müller, H. G.

AU - Lee, W P Andrew

AU - Fuchs, D.

AU - Margreiter, R.

AU - Schneeberger, S.

PY - 2009/3

Y1 - 2009/3

N2 - Human hand transplantation is complicated by skin rejection. To better define the characteristics of infiltrating cells, biopsies from human hand transplants have been investigated for expression of Foxp3 and indoleamine 2,3-dioxygenase (IDO), a key regulatory enzyme to induce T-lymphocyte unresponsiveness. A total of 104 skin biopsies taken from three bilateral hand transplant recipients over 6 years posttransplant were assessed by hematoxylin-eosin histology (graded 1-4b) and immunohistochemistry for IDO and Foxp3 according to a three-grade classification and correlated with the grade of rejection as well as time after transplantation. Overall, rejection ranged between grades 0 and 4a with an average score of 0.94. IDO was expressed in the endothelium independent of rejection. Upon rejection, IDO staining within the cellular infiltrate was significantly increased. Foxp3 in regulatory T cells was mainly found in samples undergoing severe rejection. Expression of IDO and Foxp3 compared well to each other, although the overall expression of Foxp3 was lower when compared to IDO. An increased expression of IDO as well as Foxp3 during rejection late after transplantation was observed. Characteristics of the cellular infiltrate indicate tolerogenic properties of a proportion of the cells and therefore a tendency toward self-limitation of the alloimmune response during skin rejection after hand transplantation.

AB - Human hand transplantation is complicated by skin rejection. To better define the characteristics of infiltrating cells, biopsies from human hand transplants have been investigated for expression of Foxp3 and indoleamine 2,3-dioxygenase (IDO), a key regulatory enzyme to induce T-lymphocyte unresponsiveness. A total of 104 skin biopsies taken from three bilateral hand transplant recipients over 6 years posttransplant were assessed by hematoxylin-eosin histology (graded 1-4b) and immunohistochemistry for IDO and Foxp3 according to a three-grade classification and correlated with the grade of rejection as well as time after transplantation. Overall, rejection ranged between grades 0 and 4a with an average score of 0.94. IDO was expressed in the endothelium independent of rejection. Upon rejection, IDO staining within the cellular infiltrate was significantly increased. Foxp3 in regulatory T cells was mainly found in samples undergoing severe rejection. Expression of IDO and Foxp3 compared well to each other, although the overall expression of Foxp3 was lower when compared to IDO. An increased expression of IDO as well as Foxp3 during rejection late after transplantation was observed. Characteristics of the cellular infiltrate indicate tolerogenic properties of a proportion of the cells and therefore a tendency toward self-limitation of the alloimmune response during skin rejection after hand transplantation.

UR - http://www.scopus.com/inward/record.url?scp=62849086405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62849086405&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2009.01.008

DO - 10.1016/j.transproceed.2009.01.008

M3 - Article

C2 - 19328914

AN - SCOPUS:62849086405

VL - 41

SP - 509

EP - 512

JO - Transplantation Proceedings

JF - Transplantation Proceedings

SN - 0041-1345

IS - 2

ER -