Indium-111 myosin-specific antibodies and technetium-99m pyrophosphate in the detection of acute cardiac rejection of transplanted hearts: studies in a heterotopic rat heart model

Kan Takeda, Keisuke Ueda, Ursula Scheffel, Hayden Ravert, Norman D. LaFrance, William A Baumgartner, Bruce A. Reitz, Ahvie Herskowitz, Henry N. Wagner

Research output: Contribution to journalArticle

Abstract

111In-labelled myosin-specific antibodies were evaluated as an indicator of early changes in acute rejection in a rat heart heterotopic transplant model. Uptake of antibodies was measured in allograft and isograft hearts of animals undergoing different regimens of cyclosporine treatment and compared with the uptake of technetium-99m pyrophosphate. The data were correlated with histological estimation of the severity of myocyte necrosis and signs of early rejection (venous cuffing and endocardial inflammation, indicators of perivascular infiltrate and intermyocyte extension, respectively). Myocyte necrosis in transplanted hearts was reflected by increases in technetium-99m pyrophosphate accumulation (r=0.88) but was poorly correlated with labelled antibody uptake (r=0.58). There was no positive correlation between the degree of early cardiac rejection and uptake of either of the radiopharmaceuticals: accumulation of the labelled antibodies paradoxically declined with increased histological severity scores, whereas that of technetium-99m pyrophosphate remained unchanged. Cyclosporine treatment augmented the uptake of labelled antibodies in transplanted hearts. This may be due to alterations in plasma membrane permeability brought about by the drug, resulting in a rise in antibody binding to intracellular myosin.

Original languageEnglish (US)
Pages (from-to)461-466
Number of pages6
JournalEuropean Journal Of Nuclear Medicine
Volume18
Issue number7
DOIs
StatePublished - Jul 1991

Fingerprint

Indium
Technetium
Myosins
Antibodies
Muscle Cells
Cyclosporine
Necrosis
Isografts
Radiopharmaceuticals
Allografts
diphosphoric acid
Permeability
Cell Membrane
Inflammation
Transplants
Pharmaceutical Preparations

Keywords

  • Acute cardiac rejection
  • Cardiac transplantation
  • Cyclosporine
  • Indium-111 myosin-specific antibody
  • Technetium-99m pyrophosphate

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Indium-111 myosin-specific antibodies and technetium-99m pyrophosphate in the detection of acute cardiac rejection of transplanted hearts : studies in a heterotopic rat heart model. / Takeda, Kan; Ueda, Keisuke; Scheffel, Ursula; Ravert, Hayden; LaFrance, Norman D.; Baumgartner, William A; Reitz, Bruce A.; Herskowitz, Ahvie; Wagner, Henry N.

In: European Journal Of Nuclear Medicine, Vol. 18, No. 7, 07.1991, p. 461-466.

Research output: Contribution to journalArticle

Takeda, Kan ; Ueda, Keisuke ; Scheffel, Ursula ; Ravert, Hayden ; LaFrance, Norman D. ; Baumgartner, William A ; Reitz, Bruce A. ; Herskowitz, Ahvie ; Wagner, Henry N. / Indium-111 myosin-specific antibodies and technetium-99m pyrophosphate in the detection of acute cardiac rejection of transplanted hearts : studies in a heterotopic rat heart model. In: European Journal Of Nuclear Medicine. 1991 ; Vol. 18, No. 7. pp. 461-466.
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