TY - JOUR
T1 - Indirect enzyme-linked immunosorbent assay for the quantitation of the type-specific antigen of Haemophilus influenzae b
T2 - A preliminary report
AU - Pepple, James
AU - Moxon, E. Richard
AU - Yolken, Robert H.
N1 - Funding Information:
From the Eudowood Division of lnfectious Diseases, Department of Pediatrics, The Johns Hopkins University School of Medicine. Supported in part by Grants AI 100461 (Training) and NS 12554from the National Institutes of Health, Contract No. 266-792616, NIAID, NIH, and by the Hospital for Consumptives of Maryland (Eudowood). *Reprint address: Eudowood Division of Infectious Disease, Department of Pediatrics, The Johns Hopkins Hospital, 600 N. Wolfe St., CMSC 1109, Baltimore, MD 21205.
PY - 1980/8
Y1 - 1980/8
N2 - Infections with Haemophilus influenzae type b are major causes of morbidity and mortality in young children. We developed an enzyme-linked immunosorbent assay for the diagnosis of Hib infections. The ELISA was more sensitive than both counterimmunoelectrophoresis and latex agglutination for the detection of Hib antigen in serum, urine, and cerebrospinal fluid. In addition, no false positive ELISA reactions were noted. The increased sensitivity of ELISA was especially valuable in the detection of Hib antigen following antibiotic therapy, in which case cultures are often sterile and CIE and LA can be negative. ELISA is thus a valuable tool for the early diagnosis of Hib infections.
AB - Infections with Haemophilus influenzae type b are major causes of morbidity and mortality in young children. We developed an enzyme-linked immunosorbent assay for the diagnosis of Hib infections. The ELISA was more sensitive than both counterimmunoelectrophoresis and latex agglutination for the detection of Hib antigen in serum, urine, and cerebrospinal fluid. In addition, no false positive ELISA reactions were noted. The increased sensitivity of ELISA was especially valuable in the detection of Hib antigen following antibiotic therapy, in which case cultures are often sterile and CIE and LA can be negative. ELISA is thus a valuable tool for the early diagnosis of Hib infections.
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U2 - 10.1016/S0022-3476(80)80480-X
DO - 10.1016/S0022-3476(80)80480-X
M3 - Article
C2 - 6995567
AN - SCOPUS:0018889577
SN - 0022-3476
VL - 97
SP - 233
EP - 237
JO - The Journal of pediatrics
JF - The Journal of pediatrics
IS - 2
ER -