Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes

Tiia Maaria Ketola; Martina Hanzlíková; Linda Leppänen; Manuela Raviña; Corey J. Bishop; Jordan J. Green; Arto Urtti; Helge Lemmetyinen; Marjo Yliperttula; Elina Vuorimaa-Laukkanen

doi:10.1021/jp404812a

Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes

Tiia Maaria Ketola, Martina Hanzlíková, Linda Leppänen, Manuela Raviña, Corey J. Bishop, Jordan J. Green, Arto Urtti, Helge Lemmetyinen, Marjo Yliperttula, Elina Vuorimaa-Laukkanen

School of Medicine

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The mechanism of polyethylenimine-DNA and poly(l-lysine)-DNA complex formation at pH 5.2 and 7.4 was studied by a time-resolved spectroscopic method. The formation of a polyplex core was observed to be complete at approximately N/P = 2, at which point nearly all DNA phosphate groups were bound by polymer amine groups. The data were analyzed further both by an independent binding model and by a cooperative model for multivalent ligand binding to multisubunit substrate. At pH 5.2, the polyplex formation was cooperative at all N/P ratios, whereas for pH 7.4 at N/P < 0.6 the polyplex formation followed independent binding changing to cooperative binding at higher N/Ps.

Original language	English (US)
Pages (from-to)	10405-10413
Number of pages	9
Journal	Journal of Physical Chemistry B
Volume	117
Issue number	36
DOIs	https://doi.org/10.1021/jp404812a
State	Published - Sep 12 2013

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

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10.1021/jp404812a

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title = "Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes",

abstract = "The mechanism of polyethylenimine-DNA and poly(l-lysine)-DNA complex formation at pH 5.2 and 7.4 was studied by a time-resolved spectroscopic method. The formation of a polyplex core was observed to be complete at approximately N/P = 2, at which point nearly all DNA phosphate groups were bound by polymer amine groups. The data were analyzed further both by an independent binding model and by a cooperative model for multivalent ligand binding to multisubunit substrate. At pH 5.2, the polyplex formation was cooperative at all N/P ratios, whereas for pH 7.4 at N/P < 0.6 the polyplex formation followed independent binding changing to cooperative binding at higher N/Ps.",

author = "Ketola, {Tiia Maaria} and Martina Hanzl{\'i}kov{\'a} and Linda Lepp{\"a}nen and Manuela Ravi{\~n}a and Bishop, {Corey J.} and Green, {Jordan J.} and Arto Urtti and Helge Lemmetyinen and Marjo Yliperttula and Elina Vuorimaa-Laukkanen",

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day = "12",

doi = "10.1021/jp404812a",

language = "English (US)",

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pages = "10405--10413",

journal = "Journal of Physical Chemistry B",

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T1 - Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes

AU - Ketola, Tiia Maaria

AU - Hanzlíková, Martina

AU - Leppänen, Linda

AU - Raviña, Manuela

AU - Bishop, Corey J.

AU - Green, Jordan J.

AU - Urtti, Arto

AU - Lemmetyinen, Helge

AU - Yliperttula, Marjo

AU - Vuorimaa-Laukkanen, Elina

PY - 2013/9/12

Y1 - 2013/9/12

N2 - The mechanism of polyethylenimine-DNA and poly(l-lysine)-DNA complex formation at pH 5.2 and 7.4 was studied by a time-resolved spectroscopic method. The formation of a polyplex core was observed to be complete at approximately N/P = 2, at which point nearly all DNA phosphate groups were bound by polymer amine groups. The data were analyzed further both by an independent binding model and by a cooperative model for multivalent ligand binding to multisubunit substrate. At pH 5.2, the polyplex formation was cooperative at all N/P ratios, whereas for pH 7.4 at N/P < 0.6 the polyplex formation followed independent binding changing to cooperative binding at higher N/Ps.

AB - The mechanism of polyethylenimine-DNA and poly(l-lysine)-DNA complex formation at pH 5.2 and 7.4 was studied by a time-resolved spectroscopic method. The formation of a polyplex core was observed to be complete at approximately N/P = 2, at which point nearly all DNA phosphate groups were bound by polymer amine groups. The data were analyzed further both by an independent binding model and by a cooperative model for multivalent ligand binding to multisubunit substrate. At pH 5.2, the polyplex formation was cooperative at all N/P ratios, whereas for pH 7.4 at N/P < 0.6 the polyplex formation followed independent binding changing to cooperative binding at higher N/Ps.

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Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes

Abstract

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