Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes

Tiia Maaria Ketola, Martina Hanzlíková, Linda Leppänen, Manuela Raviña, Corey J. Bishop, Jordan Green, Arto Urtti, Helge Lemmetyinen, Marjo Yliperttula, Elina Vuorimaa-Laukkanen

Research output: Contribution to journalArticle

Abstract

The mechanism of polyethylenimine-DNA and poly(l-lysine)-DNA complex formation at pH 5.2 and 7.4 was studied by a time-resolved spectroscopic method. The formation of a polyplex core was observed to be complete at approximately N/P = 2, at which point nearly all DNA phosphate groups were bound by polymer amine groups. The data were analyzed further both by an independent binding model and by a cooperative model for multivalent ligand binding to multisubunit substrate. At pH 5.2, the polyplex formation was cooperative at all N/P ratios, whereas for pH 7.4 at N/P <0.6 the polyplex formation followed independent binding changing to cooperative binding at higher N/Ps.

Original languageEnglish (US)
Pages (from-to)10405-10413
Number of pages9
JournalJournal of Physical Chemistry B
Volume117
Issue number36
DOIs
StatePublished - Sep 12 2013

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Polyethyleneimine
lysine
DNA
deoxyribonucleic acid
Amines
Polymers
Phosphates
Ligands
Substrates
amines
phosphates
ligands
DNA-lysine adduct
polymers

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Ketola, T. M., Hanzlíková, M., Leppänen, L., Raviña, M., Bishop, C. J., Green, J., ... Vuorimaa-Laukkanen, E. (2013). Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes. Journal of Physical Chemistry B, 117(36), 10405-10413. https://doi.org/10.1021/jp404812a

Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes. / Ketola, Tiia Maaria; Hanzlíková, Martina; Leppänen, Linda; Raviña, Manuela; Bishop, Corey J.; Green, Jordan; Urtti, Arto; Lemmetyinen, Helge; Yliperttula, Marjo; Vuorimaa-Laukkanen, Elina.

In: Journal of Physical Chemistry B, Vol. 117, No. 36, 12.09.2013, p. 10405-10413.

Research output: Contribution to journalArticle

Ketola, TM, Hanzlíková, M, Leppänen, L, Raviña, M, Bishop, CJ, Green, J, Urtti, A, Lemmetyinen, H, Yliperttula, M & Vuorimaa-Laukkanen, E 2013, 'Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes', Journal of Physical Chemistry B, vol. 117, no. 36, pp. 10405-10413. https://doi.org/10.1021/jp404812a
Ketola, Tiia Maaria ; Hanzlíková, Martina ; Leppänen, Linda ; Raviña, Manuela ; Bishop, Corey J. ; Green, Jordan ; Urtti, Arto ; Lemmetyinen, Helge ; Yliperttula, Marjo ; Vuorimaa-Laukkanen, Elina. / Independent versus cooperative binding in polyethylenimine-DNA and poly(l-lysine)-DNA polyplexes. In: Journal of Physical Chemistry B. 2013 ; Vol. 117, No. 36. pp. 10405-10413.
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AU - Urtti, Arto

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