Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42

Sarah E. Smith, Boris Rubinstein, Inês Mendes Pinto, Brian D. Slaughter, Jay R. Unruh, Rong Li

Research output: Contribution to journalArticle

Abstract

The ability to break symmetry and polarize through self-organization is a fundamental feature of cellular systems. A prevailing theory in yeast posits that symmetry breaking occurs via a positive feedback loop, wherein the adaptor protein Bem1 promotes local activation and accumulation of Cdc42 by directly tethering Cdc42GTP with its guanine nucleotide exchange factor (GEF) Cdc24. In this paper, we find that neither Bem1 nor the ability of Bem1 to bind Cdc42GTP is required for cell polarization. Instead, Bem1 functions primarily by boosting GEF activity, a role critical for polarization without actin filaments. In the absence of actin-based transport, polarization of Cdc42 is accomplished through Rdi1, the Cdc42 guanine nucleotide dissociation inhibitor. A mathematical model is constructed describing cell polarization as a product of distinct pathways controlling Cdc42 activation and protein localization. The model predicts a nonmonotonic dependence of cell polarization on the concentration of Rdi1 relative to that of Cdc42.

Original languageEnglish (US)
Pages (from-to)1091-1106
Number of pages16
JournalJournal of Cell Biology
Volume202
Issue number7
DOIs
StatePublished - 2013
Externally publishedYes

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Guanine Nucleotide Exchange Factors
cdc42 GTP-Binding Protein
Guanine Nucleotide Dissociation Inhibitors
Actin Cytoskeleton
Actins
Theoretical Models
Yeasts
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Smith, S. E., Rubinstein, B., Pinto, I. M., Slaughter, B. D., Unruh, J. R., & Li, R. (2013). Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42. Journal of Cell Biology, 202(7), 1091-1106. https://doi.org/10.1083/jcb.201304180

Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42. / Smith, Sarah E.; Rubinstein, Boris; Pinto, Inês Mendes; Slaughter, Brian D.; Unruh, Jay R.; Li, Rong.

In: Journal of Cell Biology, Vol. 202, No. 7, 2013, p. 1091-1106.

Research output: Contribution to journalArticle

Smith, SE, Rubinstein, B, Pinto, IM, Slaughter, BD, Unruh, JR & Li, R 2013, 'Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42', Journal of Cell Biology, vol. 202, no. 7, pp. 1091-1106. https://doi.org/10.1083/jcb.201304180
Smith, Sarah E. ; Rubinstein, Boris ; Pinto, Inês Mendes ; Slaughter, Brian D. ; Unruh, Jay R. ; Li, Rong. / Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42. In: Journal of Cell Biology. 2013 ; Vol. 202, No. 7. pp. 1091-1106.
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