Increases in cytosolic Ca2+ accompany basophil activation by eosinophil granule major basic protein

Donald W. MacGlashan, Gerald J. Gleich, Larry L. Thomas

Research output: Contribution to journalArticlepeer-review


The response of basophil cytosolic free Ca2+ concentrations ([Ca2+](i)) to stimulation by eosinophil granule major basic protein (MBP) was assessed using digital video microscopy. MBP stimulated an elevation in basophil [Ca2+](i) that, in general, developed slowly and increased monotonically. In some experiments, however, the average rise in [Ca2+](i) was biphasic, with an initial transient increase followed by a larger and more sustained elevation. At the single cell level MBP stimulation caused frequent, asynchronous oscillations in [Ca2+](i) that ranged up to 300 nM in amplitude. Chelation of extracellular Ca2+ selectively blocked the second, sustained rise in [Ca2+](i) as well as MBP-induced histamine release. Within individual experiments, the increase in [Ca2+](i) paralleled the level of histamine release. These results demonstrate that, unlike the activation of neutrophils or eosinophils, basophil activation by MBP correlates with an elevation in [Ca2+](i). Further, the MBP induced [Ca2+](i) response exhibits several features in common with the IgE-mediated [Ca2+](i) response.

Original languageEnglish (US)
Pages (from-to)37-42
Number of pages6
JournalImmunology Letters
Issue number1
StatePublished - May 1997


  • Activation
  • Basophils
  • Calcium
  • Eosinophils

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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