Increased sensitivity of lymphocyte Δ1pyrroline-5- carboxylate reductase to inhibition by proline with transformation

David Valle, R. Michael Blaese, James M. Phang

Research output: Contribution to journalArticlepeer-review


THE mitogen-induced transformation of resting lymphocytes to proliferating blast forms is associated with a marked increase in amino-acid uptake 1, protein synthesis2 and enzyme activities3. There is little information, however, on the effect of transformation on enzymes of amino-acid metabolism. We therefore examined the biosynthetic and degradative enzymes of the non-essential amino acid, proline4, in resting and transformed lymphocytes. We found a selective increase of proline bio-synthetic enzymes in association with transformation. It is interesting that transformation also produced qualitative changes in Δ1- pyrroline-5-carboxylate reductase, the enzyme which catalyses the committed step in proline synthesis.

Original languageEnglish (US)
Pages (from-to)214-216
Number of pages3
Issue number5488
StatePublished - 1975
Externally publishedYes

ASJC Scopus subject areas

  • General

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