Increased protein synthesis is necessary for the development of late preconditioning against myocardial stunning

Ali Rizvi, Xian Liang Tang, Qiu Yumin, Yu Ting Xuan, Hitoshi Takano, Asad K. Jadoon, Roberto Bolli

Research output: Contribution to journalArticle

Abstract

In phase I of this study, the rate of protein synthesis was measured by the incorporation of [3H]leucine into the protein pool in the heart of conscious rabbits. At 2 h after ischemic preconditioning (PC) with six 4-min occlusion/4-min reperfusion (O/R) cycles (group II), the [3H]leucine content in the ischemic-reperfused region was increased by 82% compared with that in controls (group I), indicating increased protein synthesis. This increase was completely abrogated by pretreatment with cycloheximide (CH; group III). In phase II, rabbits underwent six O/R cycles for three consecutive days (days 1-3). Controls (group IV) exhibited late PC against myocardial stunning on days 2 and 3. In group V, which received CH 30 min before the 1st O/R cycle on day 1 (same dose as group III), late PC against stunning on day 2 was completely abrogated. In group VI, pretreatment with CH 24 h before the 1st sequence of O/R cycles had no effect on myocardial stunning on day 1, indicating that the absence of late PC on day 2 in group V cannot be ascribed to delayed toxicity of CH. Taken together, these results demonstrate that, in the conscious rabbit, ischemic PC causes a rapid increase in myocardial protein synthesis and that this increased protein synthesis (or at least a fraction of it) is necessary for the development of the protection against myocardial stunning 24 h later. The late phase of ischemic PC is therefore dependent on the formation of new proteins in intact animals.

Original languageEnglish (US)
Pages (from-to)H874-H884
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume277
Issue number3 46-3
StatePublished - Sep 1 1999

Keywords

  • Cycloheximide
  • Myocardial ischemia
  • Myocardial reperfusion
  • Systolic wall thickening
  • [H]leucine

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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    Rizvi, A., Tang, X. L., Yumin, Q., Xuan, Y. T., Takano, H., Jadoon, A. K., & Bolli, R. (1999). Increased protein synthesis is necessary for the development of late preconditioning against myocardial stunning. American Journal of Physiology - Heart and Circulatory Physiology, 277(3 46-3), H874-H884.