Increased phosphorylation rate of intermediate filaments during mitotic arrest

Sabina I. Robinson, Barry D Nelkin, Scott Kaufmann, Bert Vogelstein

Research output: Contribution to journalArticle

Abstract

A detergent insoluble 58 000 D polypeptide is phosphorylated at an increased rate during mitotic arrest in cultured Chinese hamster ovary cells. Using one-dimensional peptide mapping, this polypeptide was identified as a phosphorylated form of intermediate filament protein (IFP). The increased rate of phosphorylation is observed with either colcemid- or vinblastine-treated mitotic cells, but is not seen with similarly treated interphase cells.

Original languageEnglish (US)
Pages (from-to)445-449
Number of pages5
JournalExperimental Cell Research
Volume133
Issue number2
DOIs
StatePublished - 1981

Fingerprint

Intermediate Filaments
Phosphorylation
Demecolcine
Intermediate Filament Proteins
Peptides
Peptide Mapping
Vinblastine
Interphase
Cricetulus
Detergents
Ovary

ASJC Scopus subject areas

  • Cell Biology

Cite this

Increased phosphorylation rate of intermediate filaments during mitotic arrest. / Robinson, Sabina I.; Nelkin, Barry D; Kaufmann, Scott; Vogelstein, Bert.

In: Experimental Cell Research, Vol. 133, No. 2, 1981, p. 445-449.

Research output: Contribution to journalArticle

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