Increased matrix metalloproteinase-2 activity induced by TGF-β1 in duct cells of human salivary gland is associated with the development of cyst formation in vivo

Masayuki Azuma, Tetsuya Tamatani, Tokuyuki Yuki, Katsumi Motegi, Mohammad Hoque, Hideo Yoshida, Mitsunobu Sato

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Proteolytic enzyme activity has been shown to be important for cyst formation. In this study, we constructed a cyst-like structure in vivo and analyzed molecular mechanisms involved in the development of the lesion. When SV40-immortalized duct cells of normal human salivary gland (NS-SV-DC) were treated with TGF-β1 at a concentration of 1 ng/ml or 5 ng/ml followed by co- inoculation with Matrigel into the backs of nude mice, they formed large cysts containing fluid when 5 ng/ml of TGF-β1 was used. Analysis of the fluid demonstrated high MMP activity. Immunohistochemical staining exhibited strong reactivity with anti-MMP-2 antibody in TGF-β1 (5 ng/ml)-treated NS- SV-DC. Northern blot analysis indicated that the expression of TGF-β1 and MMP-2 mRNAs in cells was greatly enhanced by treatment with 5 ng/ml TGF-β1. These findings suggest that the in vivo cyst formation by TGF-β1-treated cells is associated with continuous induction of MMP-2 activity.

Original languageEnglish (US)
Pages (from-to)467-473
Number of pages7
JournalJournal of Oral Pathology and Medicine
Issue number9
Publication statusPublished - Oct 1996
Externally publishedYes



  • cysts
  • MMP-2
  • TGF-β1

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Dentistry(all)

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