Increased matrix metalloproteinase-2 activity induced by TGF-β1 in duct cells of human salivary gland is associated with the development of cyst formation in vivo

Masayuki Azuma, Tetsuya Tamatani, Tokuyuki Yuki, Katsumi Motegi, Mohammad Obaidul Hoque, Hideo Yoshida, Mitsunobu Sato

Research output: Contribution to journalArticlepeer-review

Abstract

Proteolytic enzyme activity has been shown to be important for cyst formation. In this study, we constructed a cyst-like structure in vivo and analyzed molecular mechanisms involved in the development of the lesion. When SV40-immortalized duct cells of normal human salivary gland (NS-SV-DC) were treated with TGF-β1 at a concentration of 1 ng/ml or 5 ng/ml followed by co- inoculation with Matrigel into the backs of nude mice, they formed large cysts containing fluid when 5 ng/ml of TGF-β1 was used. Analysis of the fluid demonstrated high MMP activity. Immunohistochemical staining exhibited strong reactivity with anti-MMP-2 antibody in TGF-β1 (5 ng/ml)-treated NS- SV-DC. Northern blot analysis indicated that the expression of TGF-β1 and MMP-2 mRNAs in cells was greatly enhanced by treatment with 5 ng/ml TGF-β1. These findings suggest that the in vivo cyst formation by TGF-β1-treated cells is associated with continuous induction of MMP-2 activity.

Original languageEnglish (US)
Pages (from-to)467-473
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume25
Issue number9
DOIs
StatePublished - Oct 1996
Externally publishedYes

Keywords

  • MMP-2
  • TGF-β1
  • cysts

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

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