Abstract
Proteolytic enzyme activity has been shown to be important for cyst formation. In this study, we constructed a cyst-like structure in vivo and analyzed molecular mechanisms involved in the development of the lesion. When SV40-immortalized duct cells of normal human salivary gland (NS-SV-DC) were treated with TGF-β1 at a concentration of 1 ng/ml or 5 ng/ml followed by co- inoculation with Matrigel into the backs of nude mice, they formed large cysts containing fluid when 5 ng/ml of TGF-β1 was used. Analysis of the fluid demonstrated high MMP activity. Immunohistochemical staining exhibited strong reactivity with anti-MMP-2 antibody in TGF-β1 (5 ng/ml)-treated NS- SV-DC. Northern blot analysis indicated that the expression of TGF-β1 and MMP-2 mRNAs in cells was greatly enhanced by treatment with 5 ng/ml TGF-β1. These findings suggest that the in vivo cyst formation by TGF-β1-treated cells is associated with continuous induction of MMP-2 activity.
Original language | English (US) |
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Pages (from-to) | 467-473 |
Number of pages | 7 |
Journal | Journal of Oral Pathology and Medicine |
Volume | 25 |
Issue number | 9 |
DOIs | |
State | Published - Oct 1996 |
Externally published | Yes |
Keywords
- MMP-2
- TGF-β1
- cysts
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Oral Surgery
- Otorhinolaryngology
- Cancer Research
- Periodontics