Increased manganese superoxide dismutase expression suppresses the malignant phenotype of human melanoma cells

Susan L. Church, James W. Grant, Lisa A. Ridnour, Larry W. Oberley, Paul E. Swanson, Paul S. Meltzer, Jeffrey M. Trent

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction of a normal human chromosome 6 into human melanoma cell lines results in suppression of tumorigenicity. This suggests that a gene(s) on chromosome 6 controls the malignant phenotype of human melanoma. Because antioxidants can suppress the tumor-promotion phase of carcinogenesis, and because the antioxidant enzyme manganese superoxide dismutase (MnSOD) has been localized to a region of chromosome 6 frequently lost in melanomas, we have examined the effect of transfecting sense and antisense human MnSOD cDNAs into melanoma cell lines. Cell lines expressing abundant (+)-sense MnSOD-5 cDNAs significantly altered their phenotype in culture and lost their ability to form colonies in soft agar and tumors in nude mice. In contrast, the introduction of antisense MnSOD or +psv2neo had no effect on melanoma tumorigenicity. These findings indicate that stable transfection of MnSOD cDNA into melanoma cell lines exerts a biological effect that mimics that observed after introduction of an entire human chromosome 6.

Original languageEnglish (US)
Pages (from-to)3113-3117
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number7
DOIs
StatePublished - Apr 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • General

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