The Ty1 retrotransposon of Saccharomyces cerevisiae is bounded by long- terminal repeats (LTRs). We have constructed a variety of Ty1 elements in which the LTR length has been increased from the normal length of 334 bp to >2 kb. Although small insertions in the LTR have minimal effects on transposition frequency, larger insertions dramatically reduce it. Nevertheless, elements with long LTRs are incorporated into the genome at a low frequency. Most of these rare insertion events represent Ty1 tandem (head to tail) multimers.
|Original language||English (US)|
|Number of pages||12|
|State||Published - Apr 1 1997|
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