Increased in vitro airway responsiveness in sheep following repeated exposure to antigen in vivo

E. M. Wagner, S. R. Kleeberger, E. W. Spannhake, G. K. Adams

Research output: Contribution to journalArticle

Abstract

We have studied the effect of repeated in vivo antigen exposure on in vitro airway responsiveness in sensitized sheep. Fourteen sheep underwent five biweekly exposures to aerosolized Ascaris suum antigen or saline. Following this exposure regimen, the animals were killed and tracheal smooth muscle and lung parenchymal strips were prepared for in vitro studies of isometric contraction in response to histamine, methacholine, prostaglandin F(2α), and a thromboxane A2 analogue. No alteration in tracheal smooth muscle responsiveness was observed between saline- and antigen-exposed tissue. In contrast, by use of lung parenchymal strips as an index of peripheral airway responsiveness to histamine and a thromboxane A2 analogue (10-6 and 10-5 M) were observed in antigen exposed tissue compared with saline controls. These results demonstrate that repeated antigen exposure in vivo selectively increase the responsiveness of peripheral lung smooth muscle to certain chemical mediators of anaphylaxis.

Original languageEnglish (US)
Pages (from-to)1874-1878
Number of pages5
JournalJournal of applied physiology
Volume59
Issue number6
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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