Increased frequency of P-glycoprotein gene amplification in colchicine- resistant rat-1 clones transformed by v-src

Ti Lin, Jeffrey M. Trent, Dina Milliken, David S. Shimm, Robert Donaldson, Anna B. Hill

Research output: Contribution to journalArticlepeer-review

Abstract

A rat fibroblast cell line (Rat-1) carrying a temperature-sensitive mutation of v-src was used to determine whether inducible cellular transformation altered the ability of cells to amplify the p-glycoprotein gene in response to colchicine selection. Transformed and nontransformed Rat- 1 fibroblasts selected under 4 times the LD50 generated the same number of colchicine-resistant colonies. We next examined colchicine-resistant colonies derived from transformed cells and compared them to colchicine-resistant colonies derived from nontransformed cells. When Rat-1 cells were selected at 35°C (transforming temperature), 7 out of 7 clones exhibited a 3- to 5-fold p-glycoprotein gene amplification. These results contrasted to those found at the nontransforming temperature (40°C); none of the 8 colchicine-resistant clones examined had amplified the p-glycoprotein gene. Thus in Rat-1 cells carrying a temperature-sensitive v-src gene, p-glycoprotein gene amplification was observed at a high frequency only in transformed clones selected at the temperature permissive for v-src activity.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalCancer Genetics and Cytogenetics
Volume96
Issue number2
DOIs
StatePublished - Jul 15 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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