Increased frequency of P-glycoprotein gene amplification in colchicine- resistant rat-1 clones transformed by v-src

Ti Lin; Jeffrey M. Trent; Dina Milliken; David S. Shimm; Robert Donaldson; Anna B. Hill

doi:10.1016/S0165-4608(96)00324-X

Increased frequency of P-glycoprotein gene amplification in colchicine- resistant rat-1 clones transformed by v-src

Ti Lin, Jeffrey M. Trent, Dina Milliken, David S. Shimm, Robert Donaldson, Anna B. Hill

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A rat fibroblast cell line (Rat-1) carrying a temperature-sensitive mutation of v-src was used to determine whether inducible cellular transformation altered the ability of cells to amplify the p-glycoprotein gene in response to colchicine selection. Transformed and nontransformed Rat- 1 fibroblasts selected under 4 times the LD₅₀ generated the same number of colchicine-resistant colonies. We next examined colchicine-resistant colonies derived from transformed cells and compared them to colchicine-resistant colonies derived from nontransformed cells. When Rat-1 cells were selected at 35°C (transforming temperature), 7 out of 7 clones exhibited a 3- to 5-fold p-glycoprotein gene amplification. These results contrasted to those found at the nontransforming temperature (40°C); none of the 8 colchicine-resistant clones examined had amplified the p-glycoprotein gene. Thus in Rat-1 cells carrying a temperature-sensitive v-src gene, p-glycoprotein gene amplification was observed at a high frequency only in transformed clones selected at the temperature permissive for v-src activity.

Original language	English (US)
Pages (from-to)	157-165
Number of pages	9
Journal	Cancer Genetics and Cytogenetics
Volume	96
Issue number	2
DOIs	https://doi.org/10.1016/S0165-4608(96)00324-X
State	Published - Jul 15 1997
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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title = "Increased frequency of P-glycoprotein gene amplification in colchicine- resistant rat-1 clones transformed by v-src",

abstract = "A rat fibroblast cell line (Rat-1) carrying a temperature-sensitive mutation of v-src was used to determine whether inducible cellular transformation altered the ability of cells to amplify the p-glycoprotein gene in response to colchicine selection. Transformed and nontransformed Rat- 1 fibroblasts selected under 4 times the LD50 generated the same number of colchicine-resistant colonies. We next examined colchicine-resistant colonies derived from transformed cells and compared them to colchicine-resistant colonies derived from nontransformed cells. When Rat-1 cells were selected at 35°C (transforming temperature), 7 out of 7 clones exhibited a 3- to 5-fold p-glycoprotein gene amplification. These results contrasted to those found at the nontransforming temperature (40°C); none of the 8 colchicine-resistant clones examined had amplified the p-glycoprotein gene. Thus in Rat-1 cells carrying a temperature-sensitive v-src gene, p-glycoprotein gene amplification was observed at a high frequency only in transformed clones selected at the temperature permissive for v-src activity.",

author = "Ti Lin and Trent, {Jeffrey M.} and Dina Milliken and Shimm, {David S.} and Robert Donaldson and Hill, {Anna B.}",

note = "Funding Information: We would like to thank Dr. Robert Schimke and Dr. David Smouse for a critical reading of this manuscript. We thank Dr. Joan Brugge for providing mAb 327 and the cloned c-src marker. This work was supported in part by grant CA47547 from the National Cancer Institute (A.B.H.) and by the Cancer Center Support (CORE) Grant CA 23074 to the University of Arizona.",

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T1 - Increased frequency of P-glycoprotein gene amplification in colchicine- resistant rat-1 clones transformed by v-src

AU - Lin, Ti

AU - Trent, Jeffrey M.

AU - Milliken, Dina

AU - Shimm, David S.

AU - Donaldson, Robert

AU - Hill, Anna B.

N1 - Funding Information: We would like to thank Dr. Robert Schimke and Dr. David Smouse for a critical reading of this manuscript. We thank Dr. Joan Brugge for providing mAb 327 and the cloned c-src marker. This work was supported in part by grant CA47547 from the National Cancer Institute (A.B.H.) and by the Cancer Center Support (CORE) Grant CA 23074 to the University of Arizona.

PY - 1997/7/15

Y1 - 1997/7/15

N2 - A rat fibroblast cell line (Rat-1) carrying a temperature-sensitive mutation of v-src was used to determine whether inducible cellular transformation altered the ability of cells to amplify the p-glycoprotein gene in response to colchicine selection. Transformed and nontransformed Rat- 1 fibroblasts selected under 4 times the LD50 generated the same number of colchicine-resistant colonies. We next examined colchicine-resistant colonies derived from transformed cells and compared them to colchicine-resistant colonies derived from nontransformed cells. When Rat-1 cells were selected at 35°C (transforming temperature), 7 out of 7 clones exhibited a 3- to 5-fold p-glycoprotein gene amplification. These results contrasted to those found at the nontransforming temperature (40°C); none of the 8 colchicine-resistant clones examined had amplified the p-glycoprotein gene. Thus in Rat-1 cells carrying a temperature-sensitive v-src gene, p-glycoprotein gene amplification was observed at a high frequency only in transformed clones selected at the temperature permissive for v-src activity.

AB - A rat fibroblast cell line (Rat-1) carrying a temperature-sensitive mutation of v-src was used to determine whether inducible cellular transformation altered the ability of cells to amplify the p-glycoprotein gene in response to colchicine selection. Transformed and nontransformed Rat- 1 fibroblasts selected under 4 times the LD50 generated the same number of colchicine-resistant colonies. We next examined colchicine-resistant colonies derived from transformed cells and compared them to colchicine-resistant colonies derived from nontransformed cells. When Rat-1 cells were selected at 35°C (transforming temperature), 7 out of 7 clones exhibited a 3- to 5-fold p-glycoprotein gene amplification. These results contrasted to those found at the nontransforming temperature (40°C); none of the 8 colchicine-resistant clones examined had amplified the p-glycoprotein gene. Thus in Rat-1 cells carrying a temperature-sensitive v-src gene, p-glycoprotein gene amplification was observed at a high frequency only in transformed clones selected at the temperature permissive for v-src activity.

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Increased frequency of P-glycoprotein gene amplification in colchicine- resistant rat-1 clones transformed by v-src

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