Increased expression of phosphorylated c-Jun amino-terminal kinase and phosphorylated c-Jun in human cerebral aneurysms: Role of the c-Jun amino-terminal kinase/c-Jun pathway in apoptosis of vascular walls

Yasushi Takagi, Masatsune Ishikawa, Kazuhiko Nozaki, Shinichi Yoshimura, Nobuo Hashimoto, J. Paul Elliott, Issam A. Awad, Gary K. Steinberg, R. Loch Macdonald, Maciej S. Lesniak, Daniele Rigamonti

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: Vascular remodeling via apoptotic mechanisms is an important factor in vascular diseases. c-Jun amino-terminal kinase (JNK) is a member of the mitogen-activated protein kinase family and initiates apoptosis mainly via phosphorylation of the c-Jun transcription factor. We performed this study to clarify the roles of the JNK/c-Jun pathway and apoptosis in the pathogenesis of cerebral aneurysms. METHODS: Cerebral aneurysms from 12 patients and control vessels from 5 patients were studied. We analyzed the expression of phosphorylated JNK and phosphorylated c-Jun in cerebral aneurysms by using immunohistochemical methods. RESULTS: Immunoreactivity for phosphorylated JNK and phosphorylated c-Jun was increased in the vascular walls of the cerebral aneurysms studied. Immunoreactivity for single-stranded deoxyribonucleic acid (a marker of deoxyribonucleic acid damage) was also increased in aneurysmal tissue, compared with control vessels, and was colocalized with that for phosphorylated JNK and phosphorylated c-Jun in smooth muscle cells. CONCLUSION: These observations may lead to better understanding of the role of the JNK/c-Jun pathway in the development of cerebral aneurysms and to new strategies for treatment.

Original languageEnglish (US)
Pages (from-to)997-1004
Number of pages8
JournalNeurosurgery
Volume51
Issue number4
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Keywords

  • Apoptosis
  • Cerebral aneurysm
  • Deoxyribonucleic acid fragmentation
  • Vascular remodeling
  • c-Jun
  • c-Jun amino-terminal kinase

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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