Increased expression of arginase II in human diabetic corpus cavernosum: In diabetic-associated erectile dysfunction

Trinity J. Bivalacqua, Wayne J.G. Hellstrom, Philip J. Kadowitz, Hunter C. Champion

Research output: Contribution to journalArticlepeer-review

Abstract

Nitric oxide (NO) is the principal mediator of penile erection. NO is synthesized by nitric oxide synthase (NOS). It has been well documented that the major causative factor contributing to erectile dysfunction in diabetic patients is the reduction in the amount of NO synthesis in the corpora cavernosa of the penis resulting in alterations of normal penile homeostasis. Arginase is an enzyme that shares a common substrate with NOS, thus arginase may downregulate NO production by competing with NOS for this substrate, L-arginine. The purpose of the present study was to compare arginase gene expression, protein levels, and enzyme activity in diabetic human cavernosal tissue. When compared to normal human cavernosal tissue, diabetic corpus cavernosum from humans with erectile dysfunction had higher levels of arginase II protein, gene expression, and enzyme activity. In contrast, gene expression and protein levels of arginase I were not significantly different in diabetic cavernosal tissue when compared to control tissue. The reduced ability of diabetic tissue to convert L-arginine to L-citrulline via nitric oxide synthase was reversed by the selective inhibition of arginase by 2(S)-amino-6-boronohexanoic acid (ABH). These data suggest that the increased expression of arginase II in diabetic cavernosal tissue may contribute to the erectile dysfunction associated with this common disease process and may play a role in other manifestations of diabetic disease in which nitric oxide production is decreased.

Original languageEnglish (US)
Pages (from-to)923-927
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume283
Issue number4
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • Arginase
  • Corpora cavernosa
  • Diabetes
  • Erectile dysfunction
  • NOS
  • Nitric oxide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Increased expression of arginase II in human diabetic corpus cavernosum: In diabetic-associated erectile dysfunction'. Together they form a unique fingerprint.

Cite this