Increased estrogen receptor β expression correlates with decreased spine formation in the rat hippocampus

Sylwia Szymczak, Katarzyna Kalita, Jacek Jaworski, Basia Mioduszewska, Alena Savonenko, Alicja Markowska, Istvan Merchenthaler, Leszek Kaczmarek

Research output: Contribution to journalArticle

Abstract

Estrogens play an important role in the brain function acting through two receptor types, ERα and ERβ, both well-recognized as transcription factors. In this study, we investigated the ERP mRNA and protein levels in the rat hippocampus by using two in vivo models that are known to affect synapse formation. Natural estrous-proestrous cycle was used as a model in which a marked decrease in the density of hippocampal synapses was previously observed between proestrus and estrus. We have found that ERβ mRNA and protein were displayed in high levels in the estrus and in low levels in the proestrous phase. By applying kainic acid (KA) to adult rats, we demonstrated that up-regulation of ERP mRNA and protein in hippocampal CA regions was vulnerable to KA-induced excitotoxicity. Furthermore, we note a concomitant decrease of ERβ in the excitotoxicity-resistant denate gyrus that undergoes intense plastic changes, including synaptogenesis. These data suggested that decreases in ERβ expression correlated with increase in synapse formation. This notion has been tested in vitro in hippocampal cultures, in which overexpression of ERβ by means of gene transfection resulted in the lowering of the dendritic spine density that was elevated by estrogen. In summary, our results suggest that ERβ inhibits synapse formation in hippocampal neurons.

Original languageEnglish (US)
Pages (from-to)453-463
Number of pages11
JournalHippocampus
Volume16
Issue number5
DOIs
StatePublished - 2006

Fingerprint

Estrogen Receptors
Synapses
Hippocampus
Spine
Kainic Acid
Estrus
Messenger RNA
Estrogens
Proestrus
Dendritic Spines
Proteins
Estrous Cycle
Transfection
Transcription Factors
Up-Regulation
Neurons
Brain
Genes

Keywords

  • Estrogen receptor β
  • Hippocampus
  • Plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Szymczak, S., Kalita, K., Jaworski, J., Mioduszewska, B., Savonenko, A., Markowska, A., ... Kaczmarek, L. (2006). Increased estrogen receptor β expression correlates with decreased spine formation in the rat hippocampus. Hippocampus, 16(5), 453-463. https://doi.org/10.1002/hipo.20172

Increased estrogen receptor β expression correlates with decreased spine formation in the rat hippocampus. / Szymczak, Sylwia; Kalita, Katarzyna; Jaworski, Jacek; Mioduszewska, Basia; Savonenko, Alena; Markowska, Alicja; Merchenthaler, Istvan; Kaczmarek, Leszek.

In: Hippocampus, Vol. 16, No. 5, 2006, p. 453-463.

Research output: Contribution to journalArticle

Szymczak, S, Kalita, K, Jaworski, J, Mioduszewska, B, Savonenko, A, Markowska, A, Merchenthaler, I & Kaczmarek, L 2006, 'Increased estrogen receptor β expression correlates with decreased spine formation in the rat hippocampus', Hippocampus, vol. 16, no. 5, pp. 453-463. https://doi.org/10.1002/hipo.20172
Szymczak, Sylwia ; Kalita, Katarzyna ; Jaworski, Jacek ; Mioduszewska, Basia ; Savonenko, Alena ; Markowska, Alicja ; Merchenthaler, Istvan ; Kaczmarek, Leszek. / Increased estrogen receptor β expression correlates with decreased spine formation in the rat hippocampus. In: Hippocampus. 2006 ; Vol. 16, No. 5. pp. 453-463.
@article{2002df235a844802997bcae8ee26bc55,
title = "Increased estrogen receptor β expression correlates with decreased spine formation in the rat hippocampus",
abstract = "Estrogens play an important role in the brain function acting through two receptor types, ERα and ERβ, both well-recognized as transcription factors. In this study, we investigated the ERP mRNA and protein levels in the rat hippocampus by using two in vivo models that are known to affect synapse formation. Natural estrous-proestrous cycle was used as a model in which a marked decrease in the density of hippocampal synapses was previously observed between proestrus and estrus. We have found that ERβ mRNA and protein were displayed in high levels in the estrus and in low levels in the proestrous phase. By applying kainic acid (KA) to adult rats, we demonstrated that up-regulation of ERP mRNA and protein in hippocampal CA regions was vulnerable to KA-induced excitotoxicity. Furthermore, we note a concomitant decrease of ERβ in the excitotoxicity-resistant denate gyrus that undergoes intense plastic changes, including synaptogenesis. These data suggested that decreases in ERβ expression correlated with increase in synapse formation. This notion has been tested in vitro in hippocampal cultures, in which overexpression of ERβ by means of gene transfection resulted in the lowering of the dendritic spine density that was elevated by estrogen. In summary, our results suggest that ERβ inhibits synapse formation in hippocampal neurons.",
keywords = "Estrogen receptor β, Hippocampus, Plasticity",
author = "Sylwia Szymczak and Katarzyna Kalita and Jacek Jaworski and Basia Mioduszewska and Alena Savonenko and Alicja Markowska and Istvan Merchenthaler and Leszek Kaczmarek",
year = "2006",
doi = "10.1002/hipo.20172",
language = "English (US)",
volume = "16",
pages = "453--463",
journal = "Hippocampus",
issn = "1050-9631",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - Increased estrogen receptor β expression correlates with decreased spine formation in the rat hippocampus

AU - Szymczak, Sylwia

AU - Kalita, Katarzyna

AU - Jaworski, Jacek

AU - Mioduszewska, Basia

AU - Savonenko, Alena

AU - Markowska, Alicja

AU - Merchenthaler, Istvan

AU - Kaczmarek, Leszek

PY - 2006

Y1 - 2006

N2 - Estrogens play an important role in the brain function acting through two receptor types, ERα and ERβ, both well-recognized as transcription factors. In this study, we investigated the ERP mRNA and protein levels in the rat hippocampus by using two in vivo models that are known to affect synapse formation. Natural estrous-proestrous cycle was used as a model in which a marked decrease in the density of hippocampal synapses was previously observed between proestrus and estrus. We have found that ERβ mRNA and protein were displayed in high levels in the estrus and in low levels in the proestrous phase. By applying kainic acid (KA) to adult rats, we demonstrated that up-regulation of ERP mRNA and protein in hippocampal CA regions was vulnerable to KA-induced excitotoxicity. Furthermore, we note a concomitant decrease of ERβ in the excitotoxicity-resistant denate gyrus that undergoes intense plastic changes, including synaptogenesis. These data suggested that decreases in ERβ expression correlated with increase in synapse formation. This notion has been tested in vitro in hippocampal cultures, in which overexpression of ERβ by means of gene transfection resulted in the lowering of the dendritic spine density that was elevated by estrogen. In summary, our results suggest that ERβ inhibits synapse formation in hippocampal neurons.

AB - Estrogens play an important role in the brain function acting through two receptor types, ERα and ERβ, both well-recognized as transcription factors. In this study, we investigated the ERP mRNA and protein levels in the rat hippocampus by using two in vivo models that are known to affect synapse formation. Natural estrous-proestrous cycle was used as a model in which a marked decrease in the density of hippocampal synapses was previously observed between proestrus and estrus. We have found that ERβ mRNA and protein were displayed in high levels in the estrus and in low levels in the proestrous phase. By applying kainic acid (KA) to adult rats, we demonstrated that up-regulation of ERP mRNA and protein in hippocampal CA regions was vulnerable to KA-induced excitotoxicity. Furthermore, we note a concomitant decrease of ERβ in the excitotoxicity-resistant denate gyrus that undergoes intense plastic changes, including synaptogenesis. These data suggested that decreases in ERβ expression correlated with increase in synapse formation. This notion has been tested in vitro in hippocampal cultures, in which overexpression of ERβ by means of gene transfection resulted in the lowering of the dendritic spine density that was elevated by estrogen. In summary, our results suggest that ERβ inhibits synapse formation in hippocampal neurons.

KW - Estrogen receptor β

KW - Hippocampus

KW - Plasticity

UR - http://www.scopus.com/inward/record.url?scp=33745122220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745122220&partnerID=8YFLogxK

U2 - 10.1002/hipo.20172

DO - 10.1002/hipo.20172

M3 - Article

C2 - 16526034

AN - SCOPUS:33745122220

VL - 16

SP - 453

EP - 463

JO - Hippocampus

JF - Hippocampus

SN - 1050-9631

IS - 5

ER -