Increased activity of the endothelial Gi-2 protein may contribute to the protective role of estrogens on vascular function

C. Roos, M. A. Hales, T. N. Bamett, Nicholas Flavahan

Research output: Contribution to journalArticle

Abstract

The mechanisms underlying the protective role of estrogens on vascular function have not been clearly defined. Impaired signalling of the G1.2-protein can induce endothelial dysfunction and may contribute to the pathophysiology of arteriosclerosis. Therefore, experiments were conducted to determine whether female sex hormones may regulate the activity of this endothelial signal transduction pathway. Position-matched rings of isolated coronary arteries from male and female pigs were suspended for isometric tension recording in isolated organ chambers. Contractile responses to prostaglandin F were similar in rings from males and females. Endothelium-dependent relaxations evoked by the Gi-2-protein-dependent stimuli, serotonin or fluoride (direct activator) were increased in rings from females compared to males. Endothelial responses to Gi.2-protein-independent stimuli, ADP and A23187, were similar in rings from males and females. Endothelium-dependent relaxations were inhibited by L-NAME, a blocker of NO synthase, in rings from males and females. Western blot analysis was performed using plasma membranes of human aortic endothelial cells that had been cultured in the presence and absence of 17β-estradiol. Immunoreactive levels of the Gi.2-protein were increased by hormone treatment. Therefore, increased activity of the endothelial Gi-2 protein may contribute to the anti-arteriogenic influence of estrogens.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

Fingerprint

blood vessels
estrogens
Blood Vessels
Estrogens
Proteins
proteins
endothelium
Endothelium
Signal transduction
Dinoprost
NG-Nitroarginine Methyl Ester
Endothelial cells
Gonadal Steroid Hormones
Cell membranes
Fluorides
sex hormones
Arteriosclerosis
Nitric Oxide Synthase
Adenosine Diphosphate
Calcimycin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Increased activity of the endothelial Gi-2 protein may contribute to the protective role of estrogens on vascular function. / Roos, C.; Hales, M. A.; Bamett, T. N.; Flavahan, Nicholas.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

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