TY - JOUR
T1 - Increase in translatable mRNA for mitochondrial pyruvate carboxylase during differentiation of 3T3 preadipocytes
AU - Angus, C. William
AU - Lane, M. Daniel
AU - Rosenfeld, Philip J.
AU - Kelly, Thomas J.
N1 - Funding Information:
from the NIH, USPHS (AM14574-5 Institutes of Health Training Scientist Training Program,
PY - 1981/12/31
Y1 - 1981/12/31
N2 - During differentiation of 3T3 preadipocytes into adipocytes the activity of pyruvate carboxylase, a key lipogenic enzyme, rises about 20-fold. This increase of enzymatic activity is correlated with a comparable rise in the rate of incorporation of [35S]methionine into immunoadsorbable pyruvate carboxylase. Polyadenylated RNA, isolated from differentiated 3T3 adipocytes, directs the synthesis of pyruvate carboxylase in a messenger-dependent reticulocyte lysate translation system at a 18-fold greater rate than that isolated from undifferentiated cells. Thus, it appears that the differentiation-induced rise in the cellular level of pyruvate carboxylase results from an increased rate of carboxylase synthesis due to a rise in the level of translatable carboxylase messenger RNA.
AB - During differentiation of 3T3 preadipocytes into adipocytes the activity of pyruvate carboxylase, a key lipogenic enzyme, rises about 20-fold. This increase of enzymatic activity is correlated with a comparable rise in the rate of incorporation of [35S]methionine into immunoadsorbable pyruvate carboxylase. Polyadenylated RNA, isolated from differentiated 3T3 adipocytes, directs the synthesis of pyruvate carboxylase in a messenger-dependent reticulocyte lysate translation system at a 18-fold greater rate than that isolated from undifferentiated cells. Thus, it appears that the differentiation-induced rise in the cellular level of pyruvate carboxylase results from an increased rate of carboxylase synthesis due to a rise in the level of translatable carboxylase messenger RNA.
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U2 - 10.1016/0006-291X(81)90252-7
DO - 10.1016/0006-291X(81)90252-7
M3 - Article
C2 - 6174126
AN - SCOPUS:0019809558
SN - 0006-291X
VL - 103
SP - 1216
EP - 1222
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -