Incorporation of the HIV-1 microbicide cyanovirin-N in a food product

Ming Li, Dorothy L. Patton, Yvonne Cosgrove-Sweeney, Deena Ratner, Lisa C. Rohan, Alexander M. Cole, Patrick M. Tarwater, Phalguni Gupta, Bharat Ramratnam

Research output: Contribution to journalArticle

Abstract

An urgent need exists for HIV-1 microbicides. Here, we describe the in vivo testing of lactic acid bacteria bioengineered to secrete cyanovirin-N. We fed pigtail macaques a yogurt formulation that used bioengineered strains as a starter culture. Cyanovirin-N expression could be detected in the rectal vault during and immediately after feeding. Ex vivo viral challenge of rectal tissue biopsies revealed that peak viral burden was significantly lower in tissue obtained from experimental animals compared with control animals. Formulation of candidate compounds in lactic acid bacteria and their oral administration seems to be a feasible strategy for mucosal delivery of microbicides.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalJournal of acquired immune deficiency syndromes
Volume58
Issue number4
DOIs
StatePublished - Dec 1 2011

Keywords

  • CV-N
  • HIV-1
  • cyanovirin-N
  • lactic acid bacteria
  • microbicide
  • pigtail macaque

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

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