TY - JOUR
T1 - Incorporation of T and B epitopes of the circumsporozoite protein in a chemically defined synthetic vaccine against malaria
AU - Tam, James P.
AU - Clavijo, Pedro
AU - Lu, Yi An
AU - Nussenzweig, Victor
AU - Nussenzweig, Ruth
AU - Zavala, Fidel
PY - 1990/1/1
Y1 - 1990/1/1
N2 - We show here an effective and novel approach to engineer peptide-based vaccines using a chemically defined system, known as multiple peptide antigen systems (MAPs), To protect an inbred mouse strain from infection against rodent malaria. 10 mono- and di-epitope MAP models containing different arrangements and stoichiometry of functional B an/or T helper cell epitopes from the circumsporozoite protein of Plasmodium berghei were used to immunize A/J mice. While these mice did not respond to the mono-epitope MAP bearing only the B or T epitope, very high titers of antibody and protective immunity against sporozoite challenge were elicited by di-epitope MAPs, particularly those with the B and T epitopes in tandem and present in equimolar amounts. These results, obtained in a well-defined rodent malaria model, indicate that MAPs may overcome some of the difficulties in the development of synthetic vaccines, not only for malaria but also for other infectious diseases.
AB - We show here an effective and novel approach to engineer peptide-based vaccines using a chemically defined system, known as multiple peptide antigen systems (MAPs), To protect an inbred mouse strain from infection against rodent malaria. 10 mono- and di-epitope MAP models containing different arrangements and stoichiometry of functional B an/or T helper cell epitopes from the circumsporozoite protein of Plasmodium berghei were used to immunize A/J mice. While these mice did not respond to the mono-epitope MAP bearing only the B or T epitope, very high titers of antibody and protective immunity against sporozoite challenge were elicited by di-epitope MAPs, particularly those with the B and T epitopes in tandem and present in equimolar amounts. These results, obtained in a well-defined rodent malaria model, indicate that MAPs may overcome some of the difficulties in the development of synthetic vaccines, not only for malaria but also for other infectious diseases.
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M3 - Article
C2 - 1688609
AN - SCOPUS:0025099759
SN - 0022-1007
VL - 171
SP - 299
EP - 306
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 1
ER -