Incorporation of RG1 epitope into HPV16L1-VLP does not compromise L1-specific immunity

C. Schellenbacher, B. Huber, M. Skoll, S. Shafti-Keramat, Richard S Roden, R. Kirnbauer

Research output: Contribution to journalArticle

Abstract

The candidate pan-Human Papillomavirus (HPV) vaccine RG1-VLP are HPV16 major capsid protein L1 virus-like-particles (VLP) comprising a type-common epitope of HPV16 minor capsid protein L2 (RG1; aa17-36). Vaccinations have previously demonstrated efficacy against genital high-risk (hr), low-risk (lr) and cutaneous HPV. To compare RG1-VLP to licensed vaccines, rabbits (n = 3) were immunized thrice with 1 µg, 5 µg, 25 µg, or 125 µg of RG1-VLP or a 1/4 dose of Cervarix®. 5 µg of RG1-VLP or 16L1-VLP (Cervarix) induced comparable HPV16 capsid-reactive and neutralizing antibodies titers (62,500/12,500–62,500 or 1000/10,000). 25 µg RG1-VLP induced robust cross-neutralization titers (50–1000) against hrHPV18/31/33/45/52/58/26/70. To mimic reduced immunization schedules in adolescents, mice (n = 10) were immunized twice with RG1-VLP (5 µg) plus 18L1-VLP (5 µg). HPV16 neutralization (titers of 10,000) similar to Cervarix and Gardasil and cross-protection against hrHPV58 vaginal challenge was observed. RG1-VLP vaccination induces hrHPV16 neutralization comparable to similar doses of licensed vaccines, plus cross-neutralization to heterologous hrHPV even when combined with HPV18L1-VLP.

Original languageEnglish (US)
Pages (from-to)3529-3534
Number of pages6
JournalVaccine
Volume37
Issue number27
DOIs
StatePublished - Jun 12 2019

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ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Schellenbacher, C., Huber, B., Skoll, M., Shafti-Keramat, S., Roden, R. S., & Kirnbauer, R. (2019). Incorporation of RG1 epitope into HPV16L1-VLP does not compromise L1-specific immunity. Vaccine, 37(27), 3529-3534. https://doi.org/10.1016/j.vaccine.2019.05.011