INCORPORATION OF RADIOACTIVE SULPHATE INTO SULPHATIDE DURING MYELINATION IN CULTURES OF RAT CEREBELLUM

D. Silberberg, Joyce Benjamins, N. Herschkowitz, G. M. McKhann

Research output: Contribution to journalArticle

Abstract

Abstract— Explants of rat cerebellum obtained 12‐24 h after birth were maintained in culture in Maximow assemblies. About 90 per cent of the cultures myelinated after 10–12 days in vitro. Cultures maintained for varying periods of time were exposed to [35S]Na2SO4; labelled sulphatide was recovered from the total homogenate. Preparation of a subcellular fraction with density properties corresponding to those of myelin indicated that labelled sulphatide appeared in this fraction. Cultures which were poorly‐myelinated always exhibited a lower rate of inccrpomtion than well‐myelmated cultures from littermate animals, but the distribution of labelled sulphatide into the ‘myelin’ fraction was similar in the two groups. The rate of incorporation of [35S]Na2SO4 into total sulphatide increased with the duration of the culture, with a low level of incorporation until the seventh day in vitro, followed by a sharp increase in rate up to the 21st day. This pattern resembles that observed for rat cerebellum in vivo.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalJournal of Neurochemistry
Volume19
Issue number1
DOIs
StatePublished - Jan 1972

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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