TY - JOUR
T1 - Incorporation of carbohydrate into protein of human platelets
AU - Zieve, Philip D.
AU - Schmukler, Morton
N1 - Funding Information:
Supported by a grant HL-09102 from the National Heart Institute and an Institutional Research Grant, 5--90-IRR0556-10, to Baltimore City Hospitals.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1974/11/4
Y1 - 1974/11/4
N2 - Human platelets incorporated glucosamine and mannose into protein. Incorporation in whole or disrupted cells was reduced considerably by incubation with thrombin. The effect of thrombin appeared to be due to hydrolysis of glycoprotein. Cyanide and fluoride also markedly inhibited incorporation; concanavalin A, prostaglandin E1, aspirin, ADP, and puromycin had no effect. Neither concanavalin A, ADP or prostaglandin E1 blocked the inhibition of incorporation by thrombin. The experiments indicate that human platelets add carbohydrate onto pre-existing protein by an energy-dependent process and that this glycoprotein is hydrolyzed by thrombin.
AB - Human platelets incorporated glucosamine and mannose into protein. Incorporation in whole or disrupted cells was reduced considerably by incubation with thrombin. The effect of thrombin appeared to be due to hydrolysis of glycoprotein. Cyanide and fluoride also markedly inhibited incorporation; concanavalin A, prostaglandin E1, aspirin, ADP, and puromycin had no effect. Neither concanavalin A, ADP or prostaglandin E1 blocked the inhibition of incorporation by thrombin. The experiments indicate that human platelets add carbohydrate onto pre-existing protein by an energy-dependent process and that this glycoprotein is hydrolyzed by thrombin.
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U2 - 10.1016/0304-4165(74)90089-0
DO - 10.1016/0304-4165(74)90089-0
M3 - Article
C2 - 4425669
AN - SCOPUS:0016270972
VL - 372
SP - 225
EP - 229
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 1
ER -