Incomplete global cerebral ischemia alters platelet biology in neonatal and adult sheep

Marguerite T. Littleton-Kearney, Patricia D. Hurn, Thomas Stephen Kickler, Richard J. Traystman

Research output: Contribution to journalArticle

Abstract

Platelets are implicated as etiologic agents in cerebral ischemia and as modulators of neural injury following an ischemic insult. We examined the effects of severe, transient global ischemia on platelet aggregation during 45-min ischemia and 30-, 60-, and 120-min reperfusion in adult and neonatal lambs. We also examined postischemic platelet deposition in brain and other tissues (120-min reperfusion) using indium-111-labeled platelets. Ischemic cerebral blood flow fell to 5 ± 1 and 5 ± 2 ml·min-1·100 g-1 in lambs and sheep, respectively. During ischemia, platelet counts fell to 47.5 ± 5.1% of control (P <0.05) in lambs and 59 ± 4.9% of control in sheep (P <0.05). Ischemia depressed platelet aggregation response (P <0.01) to 4 μg collagen in lambs and sheep (20.4 ± 29.2 and 26 ± 44.7% of control, respectively). Marked platelet deposition occurred in brain and spleen in sheep, whereas significant platelet entrapment occurred only in brain in lambs. Our findings suggest that ischemia causes platelet activation and deposition in brain and noncerebral tissues.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume43
Issue number4
StatePublished - Apr 1998

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Brain Ischemia
Sheep
Blood Platelets
Ischemia
Brain
Platelet Aggregation
Reperfusion
Cerebrovascular Circulation
Indium
Platelet Activation
Platelet Count
Collagen
Spleen
Wounds and Injuries

Keywords

  • Chronological age
  • Platelet aggregation
  • Platelet entrapment

ASJC Scopus subject areas

  • Physiology

Cite this

Incomplete global cerebral ischemia alters platelet biology in neonatal and adult sheep. / Littleton-Kearney, Marguerite T.; Hurn, Patricia D.; Kickler, Thomas Stephen; Traystman, Richard J.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 43, No. 4, 04.1998.

Research output: Contribution to journalArticle

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