Incidence of chromosomal mosaicism in morphologically normal nonhuman primate preimplantation embryos

Cathérine Dupont, James Segars, Alan DeCherney, Barry D. Bavister, D. Randall Armant, Carol A. Brenner

Research output: Contribution to journalArticle

Abstract

Objective: To establish the exact rates of chromosomal mosaicism in morphologically normal rhesus macaque embryos by determining the chromosomal complement of all blastomeres. Design: Retrospective rhesus monkey IVF study. Setting: Academic laboratory and primate research center. Patient(s): Young fertile rhesus macaque females. Intervention(s): Morphologically normal in vitro-produced rhesus macaque embryos were dissociated and cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y. Main Outcome Measure(s): The incidence and extent of chromosomal mosaicism in rhesus macaque preimplantation embryos. Result(s): Seventy-seven preimplantation embryos, displaying normal morphology and development, from 17 young rhesus macaque females were analyzed. Overall, 39 embryos (50.6%) were normal, 14 embryos (18.2%) were completely abnormal, and 24 embryos (31.2%) were mosaic. Of the 226 blastomeres analyzed in the mosaic group, 110 blastomeres (48.7%) were normal. Conclusion(s): The observed rate of mosaicism in good-quality rhesus embryos resembles previously documented frequencies in poor-quality human preimplantation embryos. A high incidence of mosaicism may limit the diagnostic accuracy of preimplantation genetic diagnosis.

Original languageEnglish (US)
Pages (from-to)2545-2550
Number of pages6
JournalFertility and Sterility
Volume93
Issue number8
DOIs
StatePublished - May 15 2010
Externally publishedYes

Fingerprint

Mosaicism
Blastocyst
Macaca mulatta
Primates
Embryonic Structures
Incidence
Blastomeres
Preimplantation Diagnosis
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 13
Human Chromosomes
Fluorescence In Situ Hybridization
Color
Chromosomes
Outcome Assessment (Health Care)
Research

Keywords

  • aneuploidy
  • embryos
  • FISH
  • monkey
  • Mosaicism
  • nonhuman primate

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Incidence of chromosomal mosaicism in morphologically normal nonhuman primate preimplantation embryos. / Dupont, Cathérine; Segars, James; DeCherney, Alan; Bavister, Barry D.; Armant, D. Randall; Brenner, Carol A.

In: Fertility and Sterility, Vol. 93, No. 8, 15.05.2010, p. 2545-2550.

Research output: Contribution to journalArticle

Dupont, Cathérine ; Segars, James ; DeCherney, Alan ; Bavister, Barry D. ; Armant, D. Randall ; Brenner, Carol A. / Incidence of chromosomal mosaicism in morphologically normal nonhuman primate preimplantation embryos. In: Fertility and Sterility. 2010 ; Vol. 93, No. 8. pp. 2545-2550.
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