Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.)

Mi Ok Woo, Tae Ho Ham, Hyeon So Ji, Min Seon Choi, Wenzhu Jiang, Sang Ho Chu, Rihua Piao, Joong Hyoun Chin, Jung A. Kim, Bongsoo Park, Hak Soo Seo, Nam Soo Jwa, Susan McCouch, Hee Jong Koh

Research output: Contribution to journalArticle

Abstract

A rice genic male-sterility gene ms-h is recessive and has a pleiotropic effect on the chalky endosperm. After fine mapping, nucleotide sequencing analysis of the ms-h gene revealed a single nucleotide substitution at the 3′-splice junction of the 14th intron of the UDP-glucose pyrophosphorylase 1 (UGPase1; EC2.7.7.9) gene, which causes the expression of two mature transcripts with abnormal sizes caused by the aberrant splicing. An in vitro functional assay showed that both proteins encoded by the two abnormal transcripts have no UGPase activity. The suppression of UGPase by the introduction of a UGPase1-RNAi construct in wild-type plants nearly eliminated seed set because of the male defect, with developmental retardation similar to the ms-h mutant phenotype, whereas overexpression of UGPase1 in ms-h mutant plants restored male fertility and the transformants produced T1 seeds that segregated into normal and chalky endosperms. In addition, both phenotypes were co-segregated with the UGPase1 transgene in segregating T 1 plants, which demonstrates that UGPase1 has functional roles in both male sterility and the development of a chalky endosperm. Our results suggest that UGPase1 plays a key role in pollen development as well as seed carbohydrate metabolism.

Original languageEnglish (US)
Pages (from-to)190-204
Number of pages15
JournalPlant Journal
Volume54
Issue number2
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

Endosperm
Male Infertility
male fertility
Gene Silencing
endosperm
Seeds
Oryza sativa
inactivation
rice
Nucleotide Mapping
UTP-Glucose-1-Phosphate Uridylyltransferase
nucleotides
Genes
Phenotype
phenotype
mutants
genes
Carbohydrate Metabolism
carbohydrate metabolism
RNA Interference

Keywords

  • Chalky endosperm
  • Complementation test
  • Genic male sterility
  • Oryza sativa
  • RNAi
  • UGPase1

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.). / Woo, Mi Ok; Ham, Tae Ho; Ji, Hyeon So; Choi, Min Seon; Jiang, Wenzhu; Chu, Sang Ho; Piao, Rihua; Chin, Joong Hyoun; Kim, Jung A.; Park, Bongsoo; Seo, Hak Soo; Jwa, Nam Soo; McCouch, Susan; Koh, Hee Jong.

In: Plant Journal, Vol. 54, No. 2, 04.2008, p. 190-204.

Research output: Contribution to journalArticle

Woo, MO, Ham, TH, Ji, HS, Choi, MS, Jiang, W, Chu, SH, Piao, R, Chin, JH, Kim, JA, Park, B, Seo, HS, Jwa, NS, McCouch, S & Koh, HJ 2008, 'Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.)', Plant Journal, vol. 54, no. 2, pp. 190-204. https://doi.org/10.1111/j.1365-313X.2008.03405.x
Woo, Mi Ok ; Ham, Tae Ho ; Ji, Hyeon So ; Choi, Min Seon ; Jiang, Wenzhu ; Chu, Sang Ho ; Piao, Rihua ; Chin, Joong Hyoun ; Kim, Jung A. ; Park, Bongsoo ; Seo, Hak Soo ; Jwa, Nam Soo ; McCouch, Susan ; Koh, Hee Jong. / Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.). In: Plant Journal. 2008 ; Vol. 54, No. 2. pp. 190-204.
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abstract = "A rice genic male-sterility gene ms-h is recessive and has a pleiotropic effect on the chalky endosperm. After fine mapping, nucleotide sequencing analysis of the ms-h gene revealed a single nucleotide substitution at the 3′-splice junction of the 14th intron of the UDP-glucose pyrophosphorylase 1 (UGPase1; EC2.7.7.9) gene, which causes the expression of two mature transcripts with abnormal sizes caused by the aberrant splicing. An in vitro functional assay showed that both proteins encoded by the two abnormal transcripts have no UGPase activity. The suppression of UGPase by the introduction of a UGPase1-RNAi construct in wild-type plants nearly eliminated seed set because of the male defect, with developmental retardation similar to the ms-h mutant phenotype, whereas overexpression of UGPase1 in ms-h mutant plants restored male fertility and the transformants produced T1 seeds that segregated into normal and chalky endosperms. In addition, both phenotypes were co-segregated with the UGPase1 transgene in segregating T 1 plants, which demonstrates that UGPase1 has functional roles in both male sterility and the development of a chalky endosperm. Our results suggest that UGPase1 plays a key role in pollen development as well as seed carbohydrate metabolism.",
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