Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration

Stephen Lumayag, Caroline E. Haldin, Nicola J. Corbett, Karl J. Wahlin, Colleen Cowan, Sanja Turturro, Peter E. Larsen, Beatrix Kovacs, P. Dane Witmer, David Valle, Donald J Zack, Daniel A. Nicholson, Shunbin Xu

Research output: Contribution to journalArticle

Abstract

The microRNA-183/96/182 cluster is highly expressed in the retina and other sensory organs. To uncover its in vivo functions in the retina, we generated a knockout mouse model, designated "miR- 183CGT/GT," using a gene-trap embryonic stem cell clone. We provide evidence that inactivation of the cluster results in early-onset and progressive synaptic defects of the photoreceptors, leading to abnormalities of scotopic and photopic electroretinograms with decreased b-wave amplitude as the primary defect and progressive retinal degeneration. In addition, inactivation of the miR-183/96/182 cluster resulted in global changes in retinal gene expression, with enrichment of genes important for synaptogenesis, synaptic transmission, photoreceptormorphogenesis, and phototransduction, suggesting that the miR-183/96/182 cluster plays important roles in postnatal functional differentiation and synaptic connectivity of photoreceptors.

Original languageEnglish (US)
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number6
DOIs
StatePublished - Feb 5 2013

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Retinal Degeneration
MicroRNAs
Retina
Light Signal Transduction
Embryonic Stem Cells
Knockout Mice
Synaptic Transmission
Genes
Clone Cells
Gene Expression

ASJC Scopus subject areas

  • General

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Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration. / Lumayag, Stephen; Haldin, Caroline E.; Corbett, Nicola J.; Wahlin, Karl J.; Cowan, Colleen; Turturro, Sanja; Larsen, Peter E.; Kovacs, Beatrix; Witmer, P. Dane; Valle, David; Zack, Donald J; Nicholson, Daniel A.; Xu, Shunbin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 6, 05.02.2013.

Research output: Contribution to journalArticle

Lumayag, S, Haldin, CE, Corbett, NJ, Wahlin, KJ, Cowan, C, Turturro, S, Larsen, PE, Kovacs, B, Witmer, PD, Valle, D, Zack, DJ, Nicholson, DA & Xu, S 2013, 'Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 6. https://doi.org/10.1073/pnas.1212655110
Lumayag, Stephen ; Haldin, Caroline E. ; Corbett, Nicola J. ; Wahlin, Karl J. ; Cowan, Colleen ; Turturro, Sanja ; Larsen, Peter E. ; Kovacs, Beatrix ; Witmer, P. Dane ; Valle, David ; Zack, Donald J ; Nicholson, Daniel A. ; Xu, Shunbin. / Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 6.
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