Inactivation of the CDKN2/pl6/MTS1 Gene Is Frequently Associated with Aberrant DNA Methylation in All Common Human Cancers

James G. Herman, Rena G. Lapidus, Jean Pierre J. Issa, Nancy E. Davidson, David Sidransky, Stephen B. Baylin, Stephen B. Baylin, Adrian Merlo, Li Mao, David Sidransky

Research output: Contribution to journalArticle

Abstract

The tumor suppressor gene CDKN2/pl6/MTSl, located on chromosome 9p21, is frequently inactivated in many human cancers through homozygous deletion. Recently, we have reported another pathway of inactivation that involves loss of transcription associated with de novo methylation of a 5′ CpG island of CDKN2/pl6 in lung cancers, gliomas, and head and neck squamous cell carcinomas. We now show that this aberrant CpG island methylation also occurs frequently in cell lines of breast cancer (33%), prostate cancer (60%), renal cancer (23%), and colon cancer (92%) and is associated with loss of transcription. Primary tumors of the breast (31%) and colon (40%) also displayed de novo methylation of this CpG island. This alteration of pl6 in colon cancer was particularly striking, since inactivation does not occur through homozygous deletion in this tumor type. Our data show that in tumors, de novo methylation of the 5′ CpG island is a frequent mode of inactivation of CDKN2/pl6 and also firmly demonstrate that CDKN2/pl6 is one of the most frequently altered genes in human neoplasia.

Original languageEnglish (US)
Pages (from-to)4525-4530
Number of pages6
JournalCancer Research
Volume55
Issue number20
StatePublished - Oct 15 1995

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Herman, J. G., Lapidus, R. G., Issa, J. P. J., Davidson, N. E., Sidransky, D., Baylin, S. B., Baylin, S. B., Merlo, A., Mao, L., & Sidransky, D. (1995). Inactivation of the CDKN2/pl6/MTS1 Gene Is Frequently Associated with Aberrant DNA Methylation in All Common Human Cancers. Cancer Research, 55(20), 4525-4530.