Inactivation of Pkd1 in principal cells causes a more severe cystic kidney disease than in intercalated cells

Kalani L. Raphael, Kevin A. Strait, Peter K. Stricklett, R. Lance Miller, Raoul D. Nelson, Klaus B. Piontek, Gregory G. Germino, Donald E. Kohan

Research output: Contribution to journalArticle

Abstract

Renal cysts in autosomal dominant polycystic kidney disease arise from cells throughout the nephron, but there is an uncertainty as to whether both the intercalated cells (ICs) and principal cells (PCs) within the collecting duct give rise to cysts. To determine this, we crossed mice containing loxP sites within introns 1 and 4 of the Pkd1 gene with transgenic mice expressing Cre recombinase under control of the aquaporin-2 promoter or the B1 subunit of the proton ATPase promoter, thereby generating PC- or IC-specific knockout of Pkd1, respectively. Mice, that had Pkd1 deleted in the PCs, developed progressive cystic kidney disease evident during the first postnatal week and had an average lifespan of 8.2 weeks. There was no change in the cellular cAMP content or membrane aquaporin-2 expression in their kidneys. Cysts were present in the cortex and outer medulla but were absent in the papilla. Mice in which PKd1 was knocked out in the ICs had a very mild cystic phenotype as late as 13 weeks of age, limited to 1-2 cysts and confined to the outer rim of the kidney cortex. These mice lived to at least 1.5 years of age without evidence of early mortality. Our findings suggest that PCs are more important than ICs for cyst formation in polycystic kidney disease.

Original languageEnglish (US)
Pages (from-to)626-633
Number of pages8
JournalKidney International
Volume75
Issue number6
DOIs
StatePublished - 2009

Fingerprint

Cystic Kidney Diseases
Cysts
Aquaporin 2
Kidney
Autosomal Dominant Polycystic Kidney
Kidney Cortex
Polycystic Kidney Diseases
Nephrons
Introns
Transgenic Mice
Uncertainty
Adenosine Triphosphatases
Protons
Phenotype

Keywords

  • Collecting duct
  • Cre recombinase
  • Gene targeting
  • Polycystin-1

ASJC Scopus subject areas

  • Nephrology

Cite this

Raphael, K. L., Strait, K. A., Stricklett, P. K., Miller, R. L., Nelson, R. D., Piontek, K. B., ... Kohan, D. E. (2009). Inactivation of Pkd1 in principal cells causes a more severe cystic kidney disease than in intercalated cells. Kidney International, 75(6), 626-633. https://doi.org/10.1038/ki.2008.659

Inactivation of Pkd1 in principal cells causes a more severe cystic kidney disease than in intercalated cells. / Raphael, Kalani L.; Strait, Kevin A.; Stricklett, Peter K.; Miller, R. Lance; Nelson, Raoul D.; Piontek, Klaus B.; Germino, Gregory G.; Kohan, Donald E.

In: Kidney International, Vol. 75, No. 6, 2009, p. 626-633.

Research output: Contribution to journalArticle

Raphael, KL, Strait, KA, Stricklett, PK, Miller, RL, Nelson, RD, Piontek, KB, Germino, GG & Kohan, DE 2009, 'Inactivation of Pkd1 in principal cells causes a more severe cystic kidney disease than in intercalated cells', Kidney International, vol. 75, no. 6, pp. 626-633. https://doi.org/10.1038/ki.2008.659
Raphael, Kalani L. ; Strait, Kevin A. ; Stricklett, Peter K. ; Miller, R. Lance ; Nelson, Raoul D. ; Piontek, Klaus B. ; Germino, Gregory G. ; Kohan, Donald E. / Inactivation of Pkd1 in principal cells causes a more severe cystic kidney disease than in intercalated cells. In: Kidney International. 2009 ; Vol. 75, No. 6. pp. 626-633.
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