TY - JOUR
T1 - Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization
AU - Sieber, F.
AU - Krueger, G. J.
AU - O'Brien, J. M.
AU - Schober, S. L.
AU - Sensenbrenner, L. L.
AU - Sharkis, S. J.
PY - 1989
Y1 - 1989
N2 - The Friend virus complex was used as a model to study the effects of merocyanine 540 (MC 540)-mediated photosensitization on enveloped viruses. Simultaneous exposure to the lipophilic dye MC 540 and white light inactivated cell-free virus, cell-associated virus, and virus-transformed cells. When used under experimental conditions that are known to preserve most mature blood cells, at least some coagulation factors, and a significant portion of the pluripotent hematopoietic stem cell compartment, MC 540-mediated photosensitization reduced virus titers by ≥ 4 log and the concentration of in vitro clonogenic erythroleukemia cells by ≥5 log. Animals that received a single intravenous injection of photosensitized virus were resistant to a subsequent challenge with live virus. High sensitivity to MC 540-mediated photosensitization appears to be a property that is shared by other enveloped viruses. Thus, photosensitization mediated by MC 540 may be of benefit in the sterilization of blood products (in particular, cellular products), the production of vaccines, and selected areas of antiviral therapy.
AB - The Friend virus complex was used as a model to study the effects of merocyanine 540 (MC 540)-mediated photosensitization on enveloped viruses. Simultaneous exposure to the lipophilic dye MC 540 and white light inactivated cell-free virus, cell-associated virus, and virus-transformed cells. When used under experimental conditions that are known to preserve most mature blood cells, at least some coagulation factors, and a significant portion of the pluripotent hematopoietic stem cell compartment, MC 540-mediated photosensitization reduced virus titers by ≥ 4 log and the concentration of in vitro clonogenic erythroleukemia cells by ≥5 log. Animals that received a single intravenous injection of photosensitized virus were resistant to a subsequent challenge with live virus. High sensitivity to MC 540-mediated photosensitization appears to be a property that is shared by other enveloped viruses. Thus, photosensitization mediated by MC 540 may be of benefit in the sterilization of blood products (in particular, cellular products), the production of vaccines, and selected areas of antiviral therapy.
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U2 - 10.1182/blood.v73.1.345.bloodjournal731345
DO - 10.1182/blood.v73.1.345.bloodjournal731345
M3 - Article
C2 - 2910366
AN - SCOPUS:0024499433
SN - 0006-4971
VL - 73
SP - 345
EP - 350
JO - Blood
JF - Blood
IS - 1
ER -