Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization

F. Sieber, G. J. Krueger, J. M. O'Brien, S. L. Schober, L. L. Sensenbrenner, S. J. Sharkis

Research output: Contribution to journalArticle

Abstract

The Friend virus complex was used as a model to study the effects of merocyanine 540 (MC 540)-mediated photosensitization on enveloped viruses. Simultaneous exposure to the lipophilic dye MC 540 and white light inactivated cell-free virus, cell-associated virus, and virus-transformed cells. When used under experimental conditions that are known to preserve most mature blood cells, at least some coagulation factors, and a significant portion of the pluripotent hematopoietic stem cell compartment, MC 540-mediated photosensitization reduced virus titers by ≥ 4 log and the concentration of in vitro clonogenic erythroleukemia cells by ≥5 log. Animals that received a single intravenous injection of photosensitized virus were resistant to a subsequent challenge with live virus. High sensitivity to MC 540-mediated photosensitization appears to be a property that is shared by other enveloped viruses. Thus, photosensitization mediated by MC 540 may be of benefit in the sterilization of blood products (in particular, cellular products), the production of vaccines, and selected areas of antiviral therapy.

Original languageEnglish (US)
Pages (from-to)345-350
Number of pages6
JournalBlood
Volume73
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Friend murine leukemia virus
Photosensitivity Disorders
Leukemia, Erythroblastic, Acute
Viruses
Satellite Viruses
Pluripotent Stem Cells
Blood Coagulation Factors
Blood
Hematopoietic Stem Cells
Viral Load
Intravenous Injections
Antiviral Agents
merocyanine dye
Blood Cells
Coloring Agents
Vaccines
Stem cells
Light
Animals
Dyes

ASJC Scopus subject areas

  • Hematology

Cite this

Sieber, F., Krueger, G. J., O'Brien, J. M., Schober, S. L., Sensenbrenner, L. L., & Sharkis, S. J. (1989). Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization. Blood, 73(1), 345-350.

Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization. / Sieber, F.; Krueger, G. J.; O'Brien, J. M.; Schober, S. L.; Sensenbrenner, L. L.; Sharkis, S. J.

In: Blood, Vol. 73, No. 1, 1989, p. 345-350.

Research output: Contribution to journalArticle

Sieber, F, Krueger, GJ, O'Brien, JM, Schober, SL, Sensenbrenner, LL & Sharkis, SJ 1989, 'Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization', Blood, vol. 73, no. 1, pp. 345-350.
Sieber F, Krueger GJ, O'Brien JM, Schober SL, Sensenbrenner LL, Sharkis SJ. Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization. Blood. 1989;73(1):345-350.
Sieber, F. ; Krueger, G. J. ; O'Brien, J. M. ; Schober, S. L. ; Sensenbrenner, L. L. ; Sharkis, S. J. / Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization. In: Blood. 1989 ; Vol. 73, No. 1. pp. 345-350.
@article{10e349fd51504689a14ac3dcf2fad454,
title = "Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization",
abstract = "The Friend virus complex was used as a model to study the effects of merocyanine 540 (MC 540)-mediated photosensitization on enveloped viruses. Simultaneous exposure to the lipophilic dye MC 540 and white light inactivated cell-free virus, cell-associated virus, and virus-transformed cells. When used under experimental conditions that are known to preserve most mature blood cells, at least some coagulation factors, and a significant portion of the pluripotent hematopoietic stem cell compartment, MC 540-mediated photosensitization reduced virus titers by ≥ 4 log and the concentration of in vitro clonogenic erythroleukemia cells by ≥5 log. Animals that received a single intravenous injection of photosensitized virus were resistant to a subsequent challenge with live virus. High sensitivity to MC 540-mediated photosensitization appears to be a property that is shared by other enveloped viruses. Thus, photosensitization mediated by MC 540 may be of benefit in the sterilization of blood products (in particular, cellular products), the production of vaccines, and selected areas of antiviral therapy.",
author = "F. Sieber and Krueger, {G. J.} and O'Brien, {J. M.} and Schober, {S. L.} and Sensenbrenner, {L. L.} and Sharkis, {S. J.}",
year = "1989",
language = "English (US)",
volume = "73",
pages = "345--350",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

TY - JOUR

T1 - Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization

AU - Sieber, F.

AU - Krueger, G. J.

AU - O'Brien, J. M.

AU - Schober, S. L.

AU - Sensenbrenner, L. L.

AU - Sharkis, S. J.

PY - 1989

Y1 - 1989

N2 - The Friend virus complex was used as a model to study the effects of merocyanine 540 (MC 540)-mediated photosensitization on enveloped viruses. Simultaneous exposure to the lipophilic dye MC 540 and white light inactivated cell-free virus, cell-associated virus, and virus-transformed cells. When used under experimental conditions that are known to preserve most mature blood cells, at least some coagulation factors, and a significant portion of the pluripotent hematopoietic stem cell compartment, MC 540-mediated photosensitization reduced virus titers by ≥ 4 log and the concentration of in vitro clonogenic erythroleukemia cells by ≥5 log. Animals that received a single intravenous injection of photosensitized virus were resistant to a subsequent challenge with live virus. High sensitivity to MC 540-mediated photosensitization appears to be a property that is shared by other enveloped viruses. Thus, photosensitization mediated by MC 540 may be of benefit in the sterilization of blood products (in particular, cellular products), the production of vaccines, and selected areas of antiviral therapy.

AB - The Friend virus complex was used as a model to study the effects of merocyanine 540 (MC 540)-mediated photosensitization on enveloped viruses. Simultaneous exposure to the lipophilic dye MC 540 and white light inactivated cell-free virus, cell-associated virus, and virus-transformed cells. When used under experimental conditions that are known to preserve most mature blood cells, at least some coagulation factors, and a significant portion of the pluripotent hematopoietic stem cell compartment, MC 540-mediated photosensitization reduced virus titers by ≥ 4 log and the concentration of in vitro clonogenic erythroleukemia cells by ≥5 log. Animals that received a single intravenous injection of photosensitized virus were resistant to a subsequent challenge with live virus. High sensitivity to MC 540-mediated photosensitization appears to be a property that is shared by other enveloped viruses. Thus, photosensitization mediated by MC 540 may be of benefit in the sterilization of blood products (in particular, cellular products), the production of vaccines, and selected areas of antiviral therapy.

UR - http://www.scopus.com/inward/record.url?scp=0024499433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024499433&partnerID=8YFLogxK

M3 - Article

C2 - 2910366

AN - SCOPUS:0024499433

VL - 73

SP - 345

EP - 350

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -