Inactivation of C/EBP transcription factors specifically affects the synaptic plasticity of a common snail neuron

V. P. Nikitin, S. A. Kozyrev, Alexey Shevelkin

Research output: Contribution to journalArticle

Abstract

Our previous studies showed that the acquisition of nociceptive sensitization in common snails is accompanied by long-term facilitation of synaptic transmission in defensive behavior command neuron LPl1, this being dependent on translation and transcription processes. The characteristics of the neurochemical mechanisms of plasticity in the different sensory inputs of this nerve cell were identified. The mechanisms of induction of synaptic facilitation of the responses of neuron LPl1 to chemical sensory stimulation of the snail's head involved NMDA glutamate receptors, serotonin receptors, cAMP, and serotonin-modulated transcription-regulating protein SMP-69. The mechanisms of induction of synaptic facilitation of another sensory input of the neuron - from tactile receptors on the head - involved protein kinase C. The present study addresses the involvement of C/EBP transcription factors (CCAAT-enhancer-binding protein) in the processes of synapse-specific plasticity of neuron LPl1 during the acquisition of sensitization in snails. C/EBP was inactivated using oligonucleotides specifically binding to these proteins. The results showed that acquisition of sensitization during intracellular administration of oligonucleotides led to the selective suppression of synaptic facilitation in the responses of neuron LPl1 to chemical sensory stimulation of the snail's head. Synaptic facilitation in the responses to tactile stimulation of the head or foot developed as in neurons in control sensitized snails. It is suggested that C/EBP transcription factor is selectively involved in the mechanisms of synapse-specific plasticity of the sensory input of neuron LPl1 from chemoreceptors on the head during the acquisition of sensitization in snails.

Original languageEnglish (US)
Pages (from-to)757-762
Number of pages6
JournalNeuroscience and Behavioral Physiology
Volume35
Issue number7
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

Fingerprint

Neuronal Plasticity
Snails
Transcription Factors
Neurons
Head
Chemical Stimulation
Touch
Sensory Receptor Cells
Oligonucleotides
Synapses
CCAAT-Enhancer-Binding Proteins
Serotonin Receptors
Glutamate Receptors
N-Methyl-D-Aspartate Receptors
Synaptic Transmission
Protein Kinase C
Foot
Serotonin
Carrier Proteins
Proteins

Keywords

  • C/EBP
  • Gene
  • Mollusk
  • Neuron
  • Nociceptive sensitization
  • Synaptic plasticity
  • Transcription factors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inactivation of C/EBP transcription factors specifically affects the synaptic plasticity of a common snail neuron. / Nikitin, V. P.; Kozyrev, S. A.; Shevelkin, Alexey.

In: Neuroscience and Behavioral Physiology, Vol. 35, No. 7, 01.09.2005, p. 757-762.

Research output: Contribution to journalArticle

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