In vivo US monitoring of catheter-based vascular delivery of gene microspheres in pigs: Feasibility

Xiangying Du, Yuesong Yang, Catherine Le Visage, Hunter H. Chen, Robert DeJong, Bensheng Qiu, Danming Wang, Kam W. Leong, Ulrike M. Hamper, Xiaoming Yang

Research output: Contribution to journalArticle

Abstract

In this study, the authors tested the feasibility of using ultrasonography (US) to monitor catheter-based vascular gene microsphere delivery. Polymeric biodegradable microspheres (mean diameter, 5 μm) were prepared by using a double-emulsion technique to encapsulate DNA-plasmid-encoding green fluorescent protein (GFP) genes. With use of gene-delivery catheters, GFP microspheres were locally delivered into the left femoral arterial walls of six pigs; the contralateral arteries were not infused with microspheres and thus served as negative control vessels. The delivery procedures were monitored with high-frequency (8-15-MHz) transducer US. The effectiveness of monitoring with US was compared with the effectiveness of monitoring with immunohistochemical anti-GFP staining. A highly echogenic "star burst" sign around the entire vessel wall was seen at US and correlated with immunohistochemical findings that showed the destination of the gene microspheres. Study results demonstrate the potential of US for monitoring catheter-based vascular gene microsphere delivery in vivo.

Original languageEnglish (US)
Pages (from-to)555-559
Number of pages5
JournalRADIOLOGY
Volume228
Issue number2
DOIs
StatePublished - Aug 1 2003

Keywords

  • Animals
  • Genes and genetics
  • Microspheres
  • Ultrasound (US), experimental studies

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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