In vivo toxicity evaluation of gold-dendrimer composite nanodevices with different surface charges

Venugopalan Kasturirangan, Bindu M. Nair, Muhammed T S Kariapper, Wojciech Lesniak, Wei Tan, Remy Bizimungu, Peter Kanter, Karoly Toth, Sandra Buitrago, Youcef M. Rustum, Alan Hutson, Lajos P. Balogh, Mohamed K. Khan

Research output: Contribution to journalArticle

Abstract

Composite nanodevices (CNDs) are multifunctional nanomaterials with potential uses in cancer imaging and therapy. Poly(amidoamine) dendrimer-based composite nanodevices are important members of this group and consist of an organic dendrimer component and an incorporated inorganic component, in this case, gold. This study addresses the short-(14 days) and long-term (78 days) in vivo toxicity of generation-5 (G5; 5 nm) PAMAM dendrimer-based gold-CNDs (Au-CNDs) with varying surface charges (positive, negative and neutral) in C57BL/6J male mice. Detailed toxicological analyses of (1) body weight changes, (2) serum chemistry and (3) histopathological examination of 22 organs showed no evidence of organ injury or organ function compromise. Zeta potential of Au-CNDs showed significant change from their parent dendrimers upon gold incorporation, making the normally lethal positive surface dendrimer biologically safe. Also homeostatic mechanisms in vivo may compensate/repair toxic effects, something not seen with in vitro assays.

Original languageEnglish (US)
Pages (from-to)441-451
Number of pages11
JournalNanotoxicology
Volume7
Issue number4
DOIs
StatePublished - Jun 2013
Externally publishedYes

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Keywords

  • Biosafety
  • Composite nanodevice
  • in vivo toxicity
  • Nanoparticle
  • PAMAM dendrimer

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

Cite this

Kasturirangan, V., Nair, B. M., Kariapper, M. T. S., Lesniak, W., Tan, W., Bizimungu, R., Kanter, P., Toth, K., Buitrago, S., Rustum, Y. M., Hutson, A., Balogh, L. P., & Khan, M. K. (2013). In vivo toxicity evaluation of gold-dendrimer composite nanodevices with different surface charges. Nanotoxicology, 7(4), 441-451. https://doi.org/10.3109/17435390.2012.668570