In vivo time-lapse fluorescence imaging of individual retinal ganglion cells in mice

Mark K. Walsh, Harry A. Quigley

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a technique that permits time-lapse imaging of retinal ganglion cells (RGCs), their dendritic arbors and their axons in mammals in vivo. This technique utilizes a standard confocal laser scanning microscope, transgenic mice that express yellow fluorescent protein (YFP) in a subset of RGCs and survival anesthesia techniques. The same individual RGCs with their dendritic arbors and axons were multiply imaged in vivo in both adult and juvenile mice. Additionally, the same RGC that was imaged in vivo could then be located and imaged in fixed retinal whole mount preparations. This novel technique has many potential applications.

Original languageEnglish (US)
Pages (from-to)214-221
Number of pages8
JournalJournal of Neuroscience Methods
Volume169
Issue number1
DOIs
StatePublished - Mar 30 2008

Keywords

  • Fluorescence imaging
  • In vivo imaging
  • Retinal ganglion cell

ASJC Scopus subject areas

  • Neuroscience(all)

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