In vivo 31p nuclear magnetic resonance spectroscopy of subcutaneous 9l gliosarcoma: Effects of tumor growth and treatment with 1,3-bis(2-chloroethyl)-l-nitrosourea on tumor bioenergetics and histology

R. Grant Steen, Kathy McGovarn, Rafael J. Tamargo, S. Sunder Rajan, Janna P. Wehrle, Henry Brem, Jerry D. Glickson

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Abstract

In vivo 31P nuclear magnetic resonance spectroscopy was used to examine the bioenergetics of the rat 9L gliosarcoma during untreated growth and in response to chemotherapy with l,3-bis(2-chloroethyl)-l-nitrosourea. Tumor growth was associated with a decline in the phosphocreatine and nucleoside triphosphate resonances, consistent with an increase in tumor hypoxia during untreated growth. Following chemotherapy with 13-his(2-chloroethyl)-l -nitrosourea (10 mg/kg), tumor levels of phosphocreatine and nucleoside triphosphate rebounded while the level of inorganic phosphate in the tumor declined. Histological comparison of treated and untreated tumor sections 4 days posttreatment showed that the treated tumor had a lower proportion of necrotic cells, a higher proportion of viable cells, and a 5-fold higher level of interstitial space than the control tumor.

Original languageEnglish (US)
Pages (from-to)676-681
Number of pages6
JournalCancer Research
Volume48
Issue number3
StatePublished - Jan 1 1988

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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