In vivo 31P nuclear magnetic resonance spectroscopy of subcutaneous 9L gliosarcoma

Effects of tumor growth and treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea on tumor bioenergetics and histology

R. Grant Steen, R. J. Tamargo, Rafael J Tamargo, S. Sunder Rajan, H. Brem, Henry Brem, J. D. Glickson

Research output: Contribution to journalArticle

Abstract

In vivo 31P nuclear magnetic resonance spectroscopy was used to examine the bioenergetics of the rat 9L gliosarcoma during untreated growth and in response to chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea. Tumor growth was associated with a decline in the phosphocreatine and nucleoside triophosphate resonances, consistent with an increase in tumor hypoxia during untreated growth. Following chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea (10 mg/kg), tumor levels of phosphocreatine and nucleoside triphosphate rebounded while the level of inorganic phosphate in the tumor declined. Histological comparison of treated and untreated tumor sections 4 days posttreatment showed that the treated tumor had a lower proportion of necrotic cells, a higher proportion of viable cells, and a 5-fold higher level of interstitial space than the control tumor.

Original languageEnglish (US)
Pages (from-to)676-681
Number of pages6
JournalCancer Research
Volume48
Issue number3
StatePublished - 1988

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Gliosarcoma
Carmustine
Energy Metabolism
Histology
Magnetic Resonance Spectroscopy
Growth
Neoplasms
Phosphocreatine
Nucleosides
Therapeutics
Drug Therapy
Phosphates

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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In vivo 31P nuclear magnetic resonance spectroscopy of subcutaneous 9L gliosarcoma : Effects of tumor growth and treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea on tumor bioenergetics and histology. / Grant Steen, R.; Tamargo, R. J.; Tamargo, Rafael J; Sunder Rajan, S.; Brem, H.; Brem, Henry; Glickson, J. D.

In: Cancer Research, Vol. 48, No. 3, 1988, p. 676-681.

Research output: Contribution to journalArticle

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abstract = "In vivo 31P nuclear magnetic resonance spectroscopy was used to examine the bioenergetics of the rat 9L gliosarcoma during untreated growth and in response to chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea. Tumor growth was associated with a decline in the phosphocreatine and nucleoside triophosphate resonances, consistent with an increase in tumor hypoxia during untreated growth. Following chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea (10 mg/kg), tumor levels of phosphocreatine and nucleoside triphosphate rebounded while the level of inorganic phosphate in the tumor declined. Histological comparison of treated and untreated tumor sections 4 days posttreatment showed that the treated tumor had a lower proportion of necrotic cells, a higher proportion of viable cells, and a 5-fold higher level of interstitial space than the control tumor.",
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