In vivo studies of the long‐term 51Cr red cell survival of serologically incompatible red cell units

M. L. Baldwin, C. Barrasso, T. S. Kickler, H. Drew, M. ‐F Tsan, R. S. Shirey

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The long‐term survival of serologically incompatible red cell units was measured in five patients with antibodies to high‐frequency antigens. Initially, the survival of 1 ml of 51Cr‐labeled incompatible red cells was measured over 1 hour. After demonstrating that the 1‐hour survival times were successful (greater than 70%), each patient then received 5 ml of the same 51Cr‐labeled red cells followed by the transfusion of the remainder of the red cell unit. The long‐term T ½ Cr survival for each case was patient 1 (anti‐McCa), 15 days; patient 2 (anti‐JMH), 12 days; patient 3 (anti‐Kna), 31 days; patient 4 (anti‐McCa), 12 days; and patient 5 (anti‐Hya), 14 days. Each antibody tested in an in vitro homologous macrophage assay showed less than 5 percent phagocytosis. Anti‐JMH was the only antibody to react with IgG subclass antisera and was determined to be IgG4. The macrophage assay, IgG subclass testing, and short‐term (1 hour, 1 ml) 51Cr survival studies all indicated that the short‐term survival was good. However, only the measurement of long‐ term survival with transfused units of serologically incompatible red cells was able to determine the actual survival, and “clinical significance” of the alloantibodies. Determining the actual long‐term survival by the method described here can be of importance for patients requiring chronic red cell transfusion. 1985 AABB

Original languageEnglish (US)
Pages (from-to)34-38
Number of pages5
Issue number1
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology


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