Abstract
We introduce a diffused optical detection system based on the administration of a fluorophore–antibody conjugate to diseased tissue. The conjugate interacts with the antigens expressed by the diseased tissue, resulting in fluorescent labeling of the antigen. By combining an optical detection system with a reconstruction algorithm developed on the basis of the random-walk model, we were able to determine the position of the fluorophore (and, thus, of the diseased cells) in the tissue. We present three–dimensional reconstructions of the location of a fluorophore (FITC–fluorescein isothiocyanate) in the tongues of mice. Measurements were performed with the fluorophore embedded at various simulated depths. The simulations were performed with agarose-based gel slabs applied to the tongue as tissuelike phantoms. Reconstructed fluorophore locations agree well with the actual values.
Original language | English (US) |
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Pages (from-to) | 3073-3080 |
Number of pages | 8 |
Journal | Applied Optics |
Volume | 42 |
Issue number | 16 |
DOIs | |
State | Published - Jun 1 2003 |
Externally published | Yes |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Engineering (miscellaneous)
- Electrical and Electronic Engineering