In vivo quantitative three-dimensional localization of tumor labeled with exogenous specific fluorescence markers

Israel Gannot, Avital Garashi, Gallya Gannot, Victor Chernomordik, Amir Gandjbakhche

Research output: Contribution to journalArticlepeer-review

Abstract

We introduce a diffused optical detection system based on the administration of a fluorophore–antibody conjugate to diseased tissue. The conjugate interacts with the antigens expressed by the diseased tissue, resulting in fluorescent labeling of the antigen. By combining an optical detection system with a reconstruction algorithm developed on the basis of the random-walk model, we were able to determine the position of the fluorophore (and, thus, of the diseased cells) in the tissue. We present three–dimensional reconstructions of the location of a fluorophore (FITC–fluorescein isothiocyanate) in the tongues of mice. Measurements were performed with the fluorophore embedded at various simulated depths. The simulations were performed with agarose-based gel slabs applied to the tongue as tissuelike phantoms. Reconstructed fluorophore locations agree well with the actual values.

Original languageEnglish (US)
Pages (from-to)3073-3080
Number of pages8
JournalApplied Optics
Volume42
Issue number16
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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