In vivo olfactory model of app-induced neurodegeneration reveals a reversible cell-autonomous function

Ning Cheng, Huaibin Cai, Leonardo Belluscio

Research output: Contribution to journalArticle

Abstract

Amyloid precursor protein (APP) has long been linked to the neurodegeneration of Alzheimer's disease (AD), but the associated cell death has been difficult to capture in vivo, and the role of APP in effecting neuron loss is still unclear. Olfactory dysfunction is an early symptom of AD with amyloid pathology in the olfactory epithelium correlating well to the brain pathology of AD patients. As olfactory sensory neurons (OSNs) regenerate continuously with immature and matureOSNscoexisting in the same olfactory epithelium,wesought to use this unique system to study APP-induced neurodegeneration. Here we have developed an olfactory-based transgenic mouse model that overexpresses humanized APP containing familialADmutations (hAPP) in either mature or immature OSNs, and found that despite the absence of extracellular plaques a striking number of apoptotic neurons were detected by 3 weeks of age. Importantly, apoptosis was restricted to the specific population overexpressing hAPP, either mature or immature OSNs, sparing those without hAPP. Interestingly, we observed that this widespread neurodegeneration could be rapidly rescued by reducing hAPP expression levels in immature neurons. Together, these data argue that overexpressing hAPP alone could induce cell-autonomous apoptosis in both mature and immature neurons, challenging the notion that amyloid plaques are necessary for neurodegeneration. Furthermore, we show that hAPP-induced neurodegeneration is reversible, suggesting that AD-related neural loss could potentially be rescued. Thus, we propose that this unique in vivo model will not only help determine the mechanisms underlying AD-related neurodegeneration but also serve as a platform to test possible treatments.

Original languageEnglish (US)
Pages (from-to)13699-13704
Number of pages6
JournalJournal of Neuroscience
Volume31
Issue number39
DOIs
StatePublished - Sep 28 2011
Externally publishedYes

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Amyloid beta-Protein Precursor
Olfactory Receptor Neurons
Alzheimer Disease
Neurons
Olfactory Mucosa
Apoptosis
Pathology
Amyloid Plaques
Amyloid
Transgenic Mice
Cell Death
Brain
Population
Therapeutics

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

In vivo olfactory model of app-induced neurodegeneration reveals a reversible cell-autonomous function. / Cheng, Ning; Cai, Huaibin; Belluscio, Leonardo.

In: Journal of Neuroscience, Vol. 31, No. 39, 28.09.2011, p. 13699-13704.

Research output: Contribution to journalArticle

Cheng, Ning ; Cai, Huaibin ; Belluscio, Leonardo. / In vivo olfactory model of app-induced neurodegeneration reveals a reversible cell-autonomous function. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 39. pp. 13699-13704.
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