In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver.

Clemens Bos, Yahsou Delmas, Alexis Desmoulière, Anne Solanilla, Olivier Hauger, Christophe Grosset, Isabelle Dubus, Zoran Ivanovic, Jean Rosenbaum, Pierre Charbord, Christian Combe, Jeff W Bulte, Chrit T W Moonen, Jean Ripoche, Nicolas Grenier

Research output: Contribution to journalArticle

Abstract

PURPOSE: To evaluate in vivo magnetic resonance (MR) imaging with a conventional 1.5-T system for depiction and tracking of intravascularly injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs). MATERIALS AND METHODS: This study was conducted in accordance with French law governing animal research and met guidelines for animal care and use. Rat MSCs were labeled with SPIO and transfection agent. Relaxation rates at 1.5 T, cell viability, proliferation, differentiation capacity, and labeling stability were assessed in vitro as a function of SPIO concentration. MSCs were injected into renal arteries of healthy rats (labeled cells in four, unlabeled cells in two) and portal veins of rats treated with carbon tetrachloride to induce centrolobular liver necrosis (labeled cells and unlabeled cells in two each). Follow-up serial T2*-weighted gradient-echo MR imaging and R2* mapping were performed. MR imaging findings were compared histologically. RESULTS: SPIO labeling caused a strong R2* effect that increased linearly with iron dose; R2* increase for cells labeled for 48 hours with 50 microg of iron per milliliter was 50 sec(-1) per million cells per milliliter. R2* was proportional to iron load of cells. SPIO labeling did not affect cell viability (P > .27). Labeled cells were able to differentiate into adipocytes and osteocytes. Proliferation was substantially limited for MSCs labeled with 100 microg Fe/mL or greater. Label half-life was longer than 11 days. In normal kidneys, labeled MSCs caused signal intensity loss in renal cortex. After labeled MSC injection, diseased liver had diffuse granular appearance. Cells were detected for up to 7 days in kidney and 12 days in liver. Signal intensity loss and fading over time were confirmed with serial R2* mapping. At histologic analysis, signal intensity loss correlated with iron-loaded cells, primarily in renal glomeruli and hepatic sinusoids; immunohistochemical analysis results confirmed these cells were MSCs. CONCLUSION: MR imaging can aid in monitoring of intravascularly administered SPIO-labeled MSCs in vivo in kidney and liver. (c) RSNA, 2004.

Original languageEnglish (US)
Pages (from-to)781-789
Number of pages9
JournalRadiology
Volume233
Issue number3
StatePublished - Dec 2004
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Magnetic Resonance Imaging
Kidney
Liver
Iron
Cell Survival
Osteocytes
Carbon Tetrachloride
Renal Artery
Portal Vein
Adipocytes
Transfection
Half-Life
ferric oxide
Liver Diseases
Necrosis
Cell Proliferation
Guidelines
T-Lymphocytes
Injections

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Bos, C., Delmas, Y., Desmoulière, A., Solanilla, A., Hauger, O., Grosset, C., ... Grenier, N. (2004). In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver. Radiology, 233(3), 781-789.

In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver. / Bos, Clemens; Delmas, Yahsou; Desmoulière, Alexis; Solanilla, Anne; Hauger, Olivier; Grosset, Christophe; Dubus, Isabelle; Ivanovic, Zoran; Rosenbaum, Jean; Charbord, Pierre; Combe, Christian; Bulte, Jeff W; Moonen, Chrit T W; Ripoche, Jean; Grenier, Nicolas.

In: Radiology, Vol. 233, No. 3, 12.2004, p. 781-789.

Research output: Contribution to journalArticle

Bos, C, Delmas, Y, Desmoulière, A, Solanilla, A, Hauger, O, Grosset, C, Dubus, I, Ivanovic, Z, Rosenbaum, J, Charbord, P, Combe, C, Bulte, JW, Moonen, CTW, Ripoche, J & Grenier, N 2004, 'In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver.', Radiology, vol. 233, no. 3, pp. 781-789.
Bos C, Delmas Y, Desmoulière A, Solanilla A, Hauger O, Grosset C et al. In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver. Radiology. 2004 Dec;233(3):781-789.
Bos, Clemens ; Delmas, Yahsou ; Desmoulière, Alexis ; Solanilla, Anne ; Hauger, Olivier ; Grosset, Christophe ; Dubus, Isabelle ; Ivanovic, Zoran ; Rosenbaum, Jean ; Charbord, Pierre ; Combe, Christian ; Bulte, Jeff W ; Moonen, Chrit T W ; Ripoche, Jean ; Grenier, Nicolas. / In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver. In: Radiology. 2004 ; Vol. 233, No. 3. pp. 781-789.
@article{8a4c746a3a4b42df8ef279dae08eb44f,
title = "In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver.",
abstract = "PURPOSE: To evaluate in vivo magnetic resonance (MR) imaging with a conventional 1.5-T system for depiction and tracking of intravascularly injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs). MATERIALS AND METHODS: This study was conducted in accordance with French law governing animal research and met guidelines for animal care and use. Rat MSCs were labeled with SPIO and transfection agent. Relaxation rates at 1.5 T, cell viability, proliferation, differentiation capacity, and labeling stability were assessed in vitro as a function of SPIO concentration. MSCs were injected into renal arteries of healthy rats (labeled cells in four, unlabeled cells in two) and portal veins of rats treated with carbon tetrachloride to induce centrolobular liver necrosis (labeled cells and unlabeled cells in two each). Follow-up serial T2*-weighted gradient-echo MR imaging and R2* mapping were performed. MR imaging findings were compared histologically. RESULTS: SPIO labeling caused a strong R2* effect that increased linearly with iron dose; R2* increase for cells labeled for 48 hours with 50 microg of iron per milliliter was 50 sec(-1) per million cells per milliliter. R2* was proportional to iron load of cells. SPIO labeling did not affect cell viability (P > .27). Labeled cells were able to differentiate into adipocytes and osteocytes. Proliferation was substantially limited for MSCs labeled with 100 microg Fe/mL or greater. Label half-life was longer than 11 days. In normal kidneys, labeled MSCs caused signal intensity loss in renal cortex. After labeled MSC injection, diseased liver had diffuse granular appearance. Cells were detected for up to 7 days in kidney and 12 days in liver. Signal intensity loss and fading over time were confirmed with serial R2* mapping. At histologic analysis, signal intensity loss correlated with iron-loaded cells, primarily in renal glomeruli and hepatic sinusoids; immunohistochemical analysis results confirmed these cells were MSCs. CONCLUSION: MR imaging can aid in monitoring of intravascularly administered SPIO-labeled MSCs in vivo in kidney and liver. (c) RSNA, 2004.",
author = "Clemens Bos and Yahsou Delmas and Alexis Desmouli{\`e}re and Anne Solanilla and Olivier Hauger and Christophe Grosset and Isabelle Dubus and Zoran Ivanovic and Jean Rosenbaum and Pierre Charbord and Christian Combe and Bulte, {Jeff W} and Moonen, {Chrit T W} and Jean Ripoche and Nicolas Grenier",
year = "2004",
month = "12",
language = "English (US)",
volume = "233",
pages = "781--789",
journal = "Radiology",
issn = "0033-8419",
publisher = "Radiological Society of North America Inc.",
number = "3",

}

TY - JOUR

T1 - In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver.

AU - Bos, Clemens

AU - Delmas, Yahsou

AU - Desmoulière, Alexis

AU - Solanilla, Anne

AU - Hauger, Olivier

AU - Grosset, Christophe

AU - Dubus, Isabelle

AU - Ivanovic, Zoran

AU - Rosenbaum, Jean

AU - Charbord, Pierre

AU - Combe, Christian

AU - Bulte, Jeff W

AU - Moonen, Chrit T W

AU - Ripoche, Jean

AU - Grenier, Nicolas

PY - 2004/12

Y1 - 2004/12

N2 - PURPOSE: To evaluate in vivo magnetic resonance (MR) imaging with a conventional 1.5-T system for depiction and tracking of intravascularly injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs). MATERIALS AND METHODS: This study was conducted in accordance with French law governing animal research and met guidelines for animal care and use. Rat MSCs were labeled with SPIO and transfection agent. Relaxation rates at 1.5 T, cell viability, proliferation, differentiation capacity, and labeling stability were assessed in vitro as a function of SPIO concentration. MSCs were injected into renal arteries of healthy rats (labeled cells in four, unlabeled cells in two) and portal veins of rats treated with carbon tetrachloride to induce centrolobular liver necrosis (labeled cells and unlabeled cells in two each). Follow-up serial T2*-weighted gradient-echo MR imaging and R2* mapping were performed. MR imaging findings were compared histologically. RESULTS: SPIO labeling caused a strong R2* effect that increased linearly with iron dose; R2* increase for cells labeled for 48 hours with 50 microg of iron per milliliter was 50 sec(-1) per million cells per milliliter. R2* was proportional to iron load of cells. SPIO labeling did not affect cell viability (P > .27). Labeled cells were able to differentiate into adipocytes and osteocytes. Proliferation was substantially limited for MSCs labeled with 100 microg Fe/mL or greater. Label half-life was longer than 11 days. In normal kidneys, labeled MSCs caused signal intensity loss in renal cortex. After labeled MSC injection, diseased liver had diffuse granular appearance. Cells were detected for up to 7 days in kidney and 12 days in liver. Signal intensity loss and fading over time were confirmed with serial R2* mapping. At histologic analysis, signal intensity loss correlated with iron-loaded cells, primarily in renal glomeruli and hepatic sinusoids; immunohistochemical analysis results confirmed these cells were MSCs. CONCLUSION: MR imaging can aid in monitoring of intravascularly administered SPIO-labeled MSCs in vivo in kidney and liver. (c) RSNA, 2004.

AB - PURPOSE: To evaluate in vivo magnetic resonance (MR) imaging with a conventional 1.5-T system for depiction and tracking of intravascularly injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs). MATERIALS AND METHODS: This study was conducted in accordance with French law governing animal research and met guidelines for animal care and use. Rat MSCs were labeled with SPIO and transfection agent. Relaxation rates at 1.5 T, cell viability, proliferation, differentiation capacity, and labeling stability were assessed in vitro as a function of SPIO concentration. MSCs were injected into renal arteries of healthy rats (labeled cells in four, unlabeled cells in two) and portal veins of rats treated with carbon tetrachloride to induce centrolobular liver necrosis (labeled cells and unlabeled cells in two each). Follow-up serial T2*-weighted gradient-echo MR imaging and R2* mapping were performed. MR imaging findings were compared histologically. RESULTS: SPIO labeling caused a strong R2* effect that increased linearly with iron dose; R2* increase for cells labeled for 48 hours with 50 microg of iron per milliliter was 50 sec(-1) per million cells per milliliter. R2* was proportional to iron load of cells. SPIO labeling did not affect cell viability (P > .27). Labeled cells were able to differentiate into adipocytes and osteocytes. Proliferation was substantially limited for MSCs labeled with 100 microg Fe/mL or greater. Label half-life was longer than 11 days. In normal kidneys, labeled MSCs caused signal intensity loss in renal cortex. After labeled MSC injection, diseased liver had diffuse granular appearance. Cells were detected for up to 7 days in kidney and 12 days in liver. Signal intensity loss and fading over time were confirmed with serial R2* mapping. At histologic analysis, signal intensity loss correlated with iron-loaded cells, primarily in renal glomeruli and hepatic sinusoids; immunohistochemical analysis results confirmed these cells were MSCs. CONCLUSION: MR imaging can aid in monitoring of intravascularly administered SPIO-labeled MSCs in vivo in kidney and liver. (c) RSNA, 2004.

UR - http://www.scopus.com/inward/record.url?scp=16544365679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16544365679&partnerID=8YFLogxK

M3 - Article

C2 - 15486216

AN - SCOPUS:16544365679

VL - 233

SP - 781

EP - 789

JO - Radiology

JF - Radiology

SN - 0033-8419

IS - 3

ER -