In vivo model for chronic direct intratesticular drug administration

J. P. Jarow, D. S. Keeney, B. Robaire, L. L. Ewing, Barry R Zirkin

Research output: Contribution to journalArticle

Abstract

The efficacy and toxicity of a new method for chronic direct intratesticular drug infusion were assessed in a rat model. To this end, luteinizing hormone (LH) or buffer was infused via miniosmotic pumps for 14 days directly into the parenchyma of Copenhagen rat testes. The surgical manipulation and direct infusion of buffer did not have any apparent adverse effect upon either spermatogenesis or steroidogenesis as measured by testis weight, homogenization-resistant spermatid count, and in vitro response of the testes to a maximally stimulating concentration of LH. Histologic studies revealed only a localized inflammatory response in the testis around the Silastic tubing leading from the mini-osmotic pump to the testis. The biologic efficacy of direct infusion into the testis was assessed by determining the ability of mini-osmotic pump-infused LH to maintain steroidogenesis for 14 days in animals whose pituitary function was suppressed by simultaneous subcutaneous placement of testosterone/estradiol capsules. Steroidogenesis was found to be maintained quantitatively in testes infused with an appropriate dose of LH. At a given LH dose, the directly infused testes were found to produce fivefold more testosterone than contralateral testes and 10-fold more testosterone than testes from rats receiving systemic administration of the same dose of LH. We conclude that the miniosmotic pump system is a useful means to chronically administer a high concentration of LH, and presumably other agents, to the testis without any significant adverse effects.

Original languageEnglish (US)
Pages (from-to)456-461
Number of pages6
JournalJournal of Andrology
Volume15
Issue number5
StatePublished - 1994

Fingerprint

Testis
Luteinizing Hormone
Pharmaceutical Preparations
Testosterone
Buffers
Spermatids
Spermatogenesis
Capsules
Estradiol
Weights and Measures

Keywords

  • Male infertility
  • miniosmotic pumps
  • testicular toxicity

ASJC Scopus subject areas

  • Endocrinology

Cite this

Jarow, J. P., Keeney, D. S., Robaire, B., Ewing, L. L., & Zirkin, B. R. (1994). In vivo model for chronic direct intratesticular drug administration. Journal of Andrology, 15(5), 456-461.

In vivo model for chronic direct intratesticular drug administration. / Jarow, J. P.; Keeney, D. S.; Robaire, B.; Ewing, L. L.; Zirkin, Barry R.

In: Journal of Andrology, Vol. 15, No. 5, 1994, p. 456-461.

Research output: Contribution to journalArticle

Jarow, JP, Keeney, DS, Robaire, B, Ewing, LL & Zirkin, BR 1994, 'In vivo model for chronic direct intratesticular drug administration', Journal of Andrology, vol. 15, no. 5, pp. 456-461.
Jarow, J. P. ; Keeney, D. S. ; Robaire, B. ; Ewing, L. L. ; Zirkin, Barry R. / In vivo model for chronic direct intratesticular drug administration. In: Journal of Andrology. 1994 ; Vol. 15, No. 5. pp. 456-461.
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