In vivo measurement of T1 and T2 relaxation times in awake pigeon and rat brains at 7T

Mehdi Behroozi, Caroline Chwiesko, Felix Ströckens, Magdalena Sauvage, Xavier Helluy, Jutta Peterburs, Onur Güntürkün

Research output: Contribution to journalArticle

Abstract

Purpose: Establishment of regional longitudinal (T1) and transverse (T2) relaxation times in awake pigeons and rats at 7T field strength. Regional differences in relaxation times between species and between two different pigeon breeds (homing pigeons and Figurita pigeons) were investigated. Methods: T1 and T2 relaxation times were determined for nine functionally equivalent brain regions in awake pigeons and rats using a multiple spin-echo saturation recovery method with variable repetition time and a multi-slice/multi-echo sequence, respectively. Optimized head fixation and habituation protocols were applied to accustom animals to the scanning conditions and to minimize movement. Results: The habituation protocol successfully limited movement of the awake animals to a negligible minimum, allowing reliable measurement of T1 and T2 values within all regions of interest. Significant differences in relaxation times were found between rats and pigeons but not between different pigeon breeds. Conclusion: The obtained T1 and T2 values for awake pigeons and rats and the optimized habituation protocol will augment future MRI studies with awake animals. The differences in relaxation times observed between species underline the importance of the acquisition of T1/T2 values as reference points for specific experiments.

Original languageEnglish (US)
JournalMagnetic Resonance in Medicine
DOIs
StateAccepted/In press - 2017

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Columbidae
Brain
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Keywords

  • Avian
  • Longitudinal relaxation time
  • MRI
  • Transverse relaxation time

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Behroozi, M., Chwiesko, C., Ströckens, F., Sauvage, M., Helluy, X., Peterburs, J., & Güntürkün, O. (Accepted/In press). In vivo measurement of T1 and T2 relaxation times in awake pigeon and rat brains at 7T. Magnetic Resonance in Medicine. https://doi.org/10.1002/mrm.26722

In vivo measurement of T1 and T2 relaxation times in awake pigeon and rat brains at 7T. / Behroozi, Mehdi; Chwiesko, Caroline; Ströckens, Felix; Sauvage, Magdalena; Helluy, Xavier; Peterburs, Jutta; Güntürkün, Onur.

In: Magnetic Resonance in Medicine, 2017.

Research output: Contribution to journalArticle

Behroozi, Mehdi ; Chwiesko, Caroline ; Ströckens, Felix ; Sauvage, Magdalena ; Helluy, Xavier ; Peterburs, Jutta ; Güntürkün, Onur. / In vivo measurement of T1 and T2 relaxation times in awake pigeon and rat brains at 7T. In: Magnetic Resonance in Medicine. 2017.
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