In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction

Dara L. Kraitchman; Alan W. Heldman; Ergin Atalar; Luciano C. Amado; Bradley J. Martin; Mark F. Pittenger; Joshua M. Hare; Jeff W.M. Bulte

doi:10.1161/01.CIR.0000070931.62772.4E

In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction

Dara L. Kraitchman, Alan W. Heldman, Ergin Atalar, Luciano C. Amado, Bradley J. Martin, Mark F. Pittenger, Joshua M. Hare, Jeff W.M. Bulte

School of Medicine

Research output: Contribution to journal › Article › peer-review

620 Scopus citations

Abstract

Background - We investigated the potential of magnetic resonance imaging (MRI) to track magnetically labeled mesenchymal stem cells (MR-MSCs) in a swine myocardial infarction (MI) model. Methods and Results - Adult farm pigs (n=5) were subjected to closed-chest experimental MI. MR-MSCs (2.8 to 16×10⁷ cells) were injected intramyocardially under x-ray fluoroscopy. MRIs were obtained on a 1.5T MR scanner to demonstrate the location of the MR-MSCs and were correlated with histology. Contrast-enhanced MRI demonstrated successful injection in the infarct and serial MSC tracking was demonstrated in two animals. Conclusion - MRI tracking of MSCs is feasible and represents a preferred method for studying the engraftment of MSCs in MI.

Original language	English (US)
Pages (from-to)	2290-2293
Number of pages	4
Journal	Circulation
Volume	107
Issue number	18
DOIs	https://doi.org/10.1161/01.CIR.0000070931.62772.4E
State	Published - May 13 2003

Keywords

Cells
Contrast media
Magnetic resonance imaging
Myocardial infarction

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine
Physiology (medical)

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10.1161/01.CIR.0000070931.62772.4E

Cite this

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title = "In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction",

abstract = "Background - We investigated the potential of magnetic resonance imaging (MRI) to track magnetically labeled mesenchymal stem cells (MR-MSCs) in a swine myocardial infarction (MI) model. Methods and Results - Adult farm pigs (n=5) were subjected to closed-chest experimental MI. MR-MSCs (2.8 to 16×107 cells) were injected intramyocardially under x-ray fluoroscopy. MRIs were obtained on a 1.5T MR scanner to demonstrate the location of the MR-MSCs and were correlated with histology. Contrast-enhanced MRI demonstrated successful injection in the infarct and serial MSC tracking was demonstrated in two animals. Conclusion - MRI tracking of MSCs is feasible and represents a preferred method for studying the engraftment of MSCs in MI.",

keywords = "Cells, Contrast media, Magnetic resonance imaging, Myocardial infarction",

author = "Kraitchman, {Dara L.} and Heldman, {Alan W.} and Ergin Atalar and Amado, {Luciano C.} and Martin, {Bradley J.} and Pittenger, {Mark F.} and Hare, {Joshua M.} and Bulte, {Jeff W.M.}",

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doi = "10.1161/01.CIR.0000070931.62772.4E",

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TY - JOUR

T1 - In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction

AU - Kraitchman, Dara L.

AU - Heldman, Alan W.

AU - Atalar, Ergin

AU - Amado, Luciano C.

AU - Martin, Bradley J.

AU - Pittenger, Mark F.

AU - Hare, Joshua M.

AU - Bulte, Jeff W.M.

PY - 2003/5/13

Y1 - 2003/5/13

N2 - Background - We investigated the potential of magnetic resonance imaging (MRI) to track magnetically labeled mesenchymal stem cells (MR-MSCs) in a swine myocardial infarction (MI) model. Methods and Results - Adult farm pigs (n=5) were subjected to closed-chest experimental MI. MR-MSCs (2.8 to 16×107 cells) were injected intramyocardially under x-ray fluoroscopy. MRIs were obtained on a 1.5T MR scanner to demonstrate the location of the MR-MSCs and were correlated with histology. Contrast-enhanced MRI demonstrated successful injection in the infarct and serial MSC tracking was demonstrated in two animals. Conclusion - MRI tracking of MSCs is feasible and represents a preferred method for studying the engraftment of MSCs in MI.

AB - Background - We investigated the potential of magnetic resonance imaging (MRI) to track magnetically labeled mesenchymal stem cells (MR-MSCs) in a swine myocardial infarction (MI) model. Methods and Results - Adult farm pigs (n=5) were subjected to closed-chest experimental MI. MR-MSCs (2.8 to 16×107 cells) were injected intramyocardially under x-ray fluoroscopy. MRIs were obtained on a 1.5T MR scanner to demonstrate the location of the MR-MSCs and were correlated with histology. Contrast-enhanced MRI demonstrated successful injection in the infarct and serial MSC tracking was demonstrated in two animals. Conclusion - MRI tracking of MSCs is feasible and represents a preferred method for studying the engraftment of MSCs in MI.

KW - Cells

KW - Contrast media

KW - Magnetic resonance imaging

KW - Myocardial infarction

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JO - Circulation

JF - Circulation

IS - 18

ER -

In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction

Abstract

Keywords

ASJC Scopus subject areas

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